Simple and rapid screening method for microbial D-stereospecific peptidase and esterase

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Title
Simple and rapid screening method for microbial D-stereospecific peptidase and esterase
Author(s)
Seok Joon Kwon; Dae Heoun Baek; Seung Goo Lee; Moon Hee Sung
Bibliographic Citation
Biotechnology Techniques, vol. 13, no. 10, pp. 653-655
Publication Year
1999
Abstract
The use of a turbid plate containing Z-D-Ala-D-AlaOBzl was a convenient method for the screening of microorganisms with D-stereospecific peptidase activity as well as D-stereospecific esterase activity. This paper discusses the suitability of the developed method and its application to molecular cloning of D-stereospecific peptidase and esterase genes.
Keyword
D-amino acidsD-peptidesesterasespeptidasesstereospecificity
ISSN
0951-208X
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1008937104452
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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