Endoproteolytic processing of human parathyroid hormone in Saccharomyces cerevisiae mutants lacking genes encoding the Yap3, Mkc7, and Kex2 endoproteases

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dc.contributor.authorWon A Choi-
dc.contributor.authorHyun Ah Kang-
dc.contributor.authorBong Hyun Chung-
dc.date.accessioned2017-04-19T08:56:22Z-
dc.date.available2017-04-19T08:56:22Z-
dc.date.issued1999-
dc.identifier.issn10177825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4819-
dc.description.abstractWhen the human parathyroid hormone (hPTH) is expressed as a secretory product in S. cerevisiae, most of the secreted hPTH is internally cleaved by endoproteolytic processing. To investigate whether the yeast endoproteases such as Kex2p, Yap3p, and Mkc7p are involved in the endoproteolytic processing of hPTH in S. cerevisiae, hPTH was expressed in S. cerevisiae mutants deficient in one or two of the following well-known endoproteases such as Kex2p, Mkc7p, and Yap3p. Among these mutants, the yap3-disrupted (yap3Δ) and yap3/mkc7-disrupted (yap3Δmkc7Δ) yeasts showed a significant reduction in the extent of hPTH proteolysis. In contrast, the mkc7-disrupted (mkc7Δ) yeast did not reduce the proteolysis of hPTH as compared to the wild type. This suggests that Mkc7p is not involved in the endoproteolytic processing of hPTH. It was also found that the kex2-disrupted (kex2Δ) mutant was not able to secrete a detectable amount of hPTH.-
dc.publisherSouth Korea-
dc.titleEndoproteolytic processing of human parathyroid hormone in Saccharomyces cerevisiae mutants lacking genes encoding the Yap3, Mkc7, and Kex2 endoproteases-
dc.title.alternativeEndoproteolytic processing of human parathyroid hormone in Saccharomyces cerevisiae mutants lacking genes encoding the Yap3, Mkc7, and Kex2 endoproteases-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number5-
dc.citation.endPage682-
dc.citation.startPage679-
dc.citation.volume9-
dc.contributor.alternativeName최원아-
dc.contributor.alternativeName강현아-
dc.contributor.alternativeName정봉현-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 9, no. 5, pp. 679-682-
dc.subject.keywordHuman parathyroid hormone-
dc.subject.keywordsecretory production-
dc.subject.keywordproteolysis-
dc.subject.keywordendoproteases-
dc.subject.keywordS. cerevisiae-
dc.subject.localHuman parathyroid hormone-
dc.subject.localhuman parathyroid hormone (hPTH)-
dc.subject.localsecretory production-
dc.subject.localSecretory production-
dc.subject.localproteolysis-
dc.subject.localProteolysis-
dc.subject.localendoproteases-
dc.subject.localS. cerevisiae-
dc.description.journalClassY-
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