Discovery of D-stereospecific dipeptidase from thermophilic Bacillus sp. BCS-1 and its application for synthesis of D-amino acid-containing peptide

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Title
Discovery of D-stereospecific dipeptidase from thermophilic Bacillus sp. BCS-1 and its application for synthesis of D-amino acid-containing peptide
Author(s)
Dae Heoun Baek; Seok Joon Kwon; Jin Seo Park; Seung Goo LeeTae Ick Mheen; Moon Hee Sung
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 9, no. 5, pp. 646-649
Publication Year
1999
Abstract
A thermophilic bacterium producing D-stereospecific dipeptidase was isolated from Korean soil samples. The enzyme hydrolyzed the peptide bond between D-alanyl-D-alanine (D-Ala-D-Ala). The isolated bacterial strain was rod shaped, gram-positive, motile, and formed an endospore. Morphological and physiological characteristics suggested this microorganism a thermophilic Bacillus species, and was named as Bacillus sp. BCS-1. The production of D- stereospecific dipeptidase was growth-associated and optimal at 55°C. The enzyme was applied for the synthesis of D-amino acid-containing peptide, N- benzyloxycarbonyl-L-aspartyl-D-alanine benzyl ester (Z-L-Asp-D-AlaOBzl), as a model reaction. A thermodynamically controlled synthesis of Z-L-Asp-D-AlaOBzl was achieved in an organic solvent.
Keyword
Screeningthermophilic BacillusD-stereospecific dipeptidaseenzymatic synthesisD-amino acid-containing peptide
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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