Heterozygous ras mutations are preserved in serially passaged human tumor xenografts and established cell lines

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Title
Heterozygous ras mutations are preserved in serially passaged human tumor xenografts and established cell lines
Author(s)
Keiko Masuda; Hiroshi Kijima; Hwan Mook Kim; Sang Bae Han; Yasuyuki Ohnishi; Nobuko Sawa; Yoshiro Oshika; Tetsuji Tokunaga; Takashi Tsuchida; Yoshiyuki Abe; Toshiyasu Hamana; Hiroyuki Matsubayashi; Hitoshi Yamazaki; Norikazu Tamaoki; Yoshito Ueyama; Masato Nakamura
Bibliographic Citation
Oncology Reports, vol. 6, pp. 741-745
Publication Year
1999
Abstract
We examined c-K-ras gene point mutations in human tumor xenografts and established cell lines as markers of genetic stability. Our previous study demonstrated the stability of c-K-ras gene mutations in human primary neoplasms and their tumor xenografts through serial passages in mice. In this study, we established 27 human cell lines derived from various human tumor xenografts in nude mice. Point mutation of the c-K-ras gene at codon 12 was found in 29.6% (8/27) of the cell lines, as well as in 29.6% (8/27) of the xenografts. The eight ras-mutated cell lines were derived from corresponding tumor xenografts carrying the ras mutation. Heterozygous ras gene mutation was confirmed in seven of the eight ras-mutated cell lines, as well as their corresponding xenografts. The incidence, type and heterozygosity of the c-K-ras gene mutation showed no discrepancies between the original xenografts and the established cell lines. From these findings, we concluded that point mutation of the c-K-ras gene was very stable in human tumor xenografts and established cell lines derived from the xenografts.
Keyword
ras oncogenepoint mutationxenograftcell lineserial passage
ISSN
1021-335X
Publisher
Spandidos Publ Ltd
DOI
http://dx.doi.org/10.3892/or.6.4.741
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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