Isolation of xylanase-producing thermo-tolerant Bacillus sp. and its enzyme production = Xylanase를 생산하는 내열성 Bacillus 속 균주의 분리와 효소생산 조건

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Title
Isolation of xylanase-producing thermo-tolerant Bacillus sp. and its enzyme production = Xylanase를 생산하는 내열성 Bacillus 속 균주의 분리와 효소생산 조건
Author(s)
Young Seo Park; Mi Young Kang; Hak Gil Chang; Gwi Gun Park; Jong Back Kang; Jung Kee Lee; Tae Kwang Oh
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 27, no. 5, pp. 370-377
Publication Year
1999
Abstract
Thermo-tolerant bacterium producing the xylanase was isolated from soil and identified as Bacillus pumilus. This strain, named Bacillus pumilus TX703, was able to grow and produce xylanase at the culture temperature of 50°C. The maximum xylanase production was obtained when 1% (w/v) birchwood xylan and 1% (w/v) soytone were used as carbon source and nitrogen source, respectively. The biosynthesis of xylanase was under the catabolite repression induced by glucose in the culture medium, and it was completely inhibited in the presence of 0.2% (w/v) glucose. The maximum activity of xylanase was observed from pH 8.0 to 9.0 and from 50 to 60°C and the enzyme was highly heat-stable, whose activity remained was over 50% at 80°C, and was quite stable from pH 5.0 to 10.0.
Keyword
xylanaseBacillus pumilusenzyme production
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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