DC Field | Value | Language |
---|---|---|
dc.contributor.author | Joo Hyun Kang | - |
dc.contributor.author | Song Yub Shin | - |
dc.contributor.author | So Yun Jang | - |
dc.contributor.author | Kil Lyong Kim | - |
dc.contributor.author | Kyung Soo Hahm | - |
dc.date.accessioned | 2017-04-19T08:56:25Z | - |
dc.date.available | 2017-04-19T08:56:25Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4840 | - |
dc.description.abstract | PMAP-23 is a 23-residue antimicrobial peptide from porcine myeloid cells. In order to determine the effects of two Trp residues in positions 7 and 21 of PMAP-23 on antibacterial activity and phospholipid vesicle interacting property, two analogues in which Ala is substituted for Trp residue in position 7 or 21 were synthesized. A21-PMAP-23 exhibited reduced antibacterial activity and phospholipid vesicle disrupting activity when compared to those of PMAP-23 and A7-PMAP-23. PMAP-23 readily interacted with model lipid membrane and induced membrane destabilization. Therefore antibacterial activity induced by PMAP-23 is due to the interaction of cell membrane with peptide followed by membrane perturbation. A significant structural change on the SDS micelle was not found by Ala substitution of the Trp residue of PMAP-23. Also, there is a good correlation between hydrophobic interaction on RP-HPLC, expressed as retention time on RP-HPLC, and antibacterial activity. The vesicle titration experiment indicated that Trp residues located at near C-terminus are accessible to hydrophobic tail of phospholipid vesicle. This result suggests that the C-terminal end of PMAP-23 penetrates into the lipid bilayer in the course of the interaction with phospholipid membranes and is important for its antibacterial activity. | - |
dc.publisher | Elsevier | - |
dc.title | Effects of tryptophan residues of porcine myeloid antibacterial peptide PMAP-23 on antibiotic activity | - |
dc.title.alternative | Effects of tryptophan residues of porcine myeloid antibacterial peptide PMAP-23 on antibiotic activity | - |
dc.type | Article | - |
dc.citation.title | Biochemical and Biophysical Research Communications | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 286 | - |
dc.citation.startPage | 281 | - |
dc.citation.volume | 264 | - |
dc.contributor.affiliatedAuthor | Kil Lyong Kim | - |
dc.contributor.affiliatedAuthor | Kyung Soo Hahm | - |
dc.contributor.alternativeName | 강주현 | - |
dc.contributor.alternativeName | 신송엽 | - |
dc.contributor.alternativeName | 장소윤 | - |
dc.contributor.alternativeName | 김길룡 | - |
dc.contributor.alternativeName | 함경수 | - |
dc.identifier.bibliographicCitation | Biochemical and Biophysical Research Communications, vol. 264, pp. 281-286 | - |
dc.identifier.doi | 10.1006/bbrc.1999.1510 | - |
dc.description.journalClass | Y | - |
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