Antioxidative compounds in extracts of Acer ginnala max = 신나무 추출물의 항산화 활성물질

Abstract

To search for antioxidative compounds from plant resources, methanol extracts of 45 plant species were investigated using DPPH method. The highest activity was shown in the methanol extract of Acer ginnala(RC50 : 15μg), followed by Stewartia koreana(RC50:28μg) and Carpinus laxiflora(RC50:33μg). Two antioxidative compounds were isolated from the methanolic extract of Acer ginnala Max and identified as acertannin(2, 6-di-O-galloyl-1, 5-anhydro-D-glucitol) and gallicin (methyl-3, 4, 5-trihydroxybenzoic acid) on the basis of mass spectroscopy, 1H？and 13C？NMR data. The DPPH free radical scavenging activities of acertannin(RC50:3.5μg) and gallicin(RC50:2.8μg) were more effective than those of BHA(RC50:14μg) and α？tocopherol (RC50:12μg).