Effect of specific production rate of recombinant protein on multimerization of plasmid vector and gene expression level

Cited 0 time in scopus
Metadata Downloads
Title
Effect of specific production rate of recombinant protein on multimerization of plasmid vector and gene expression level
Author(s)
Vibhor Saraswat; Dae Young Kim; Jee Won Lee; Young Hoon Park
Bibliographic Citation
FEMS Microbiology Letters, vol. 179, pp. 367-373
Publication Year
1999
Abstract
In fed-batch cultures of recombinant Escherichia coli BL21(DE3)[pT7-G3IL2] at high cell concentration, the post-induction specific growth rate was carefully regulated by controlled medium feed to maximize the synthesis level of recombinant fusion interleukin-2, G3.IL-2. A maximum concentration of G3.IL-2 (11.25 g l-1) was achieved in the induced recombinant culture growing at the rate of 0.056 h-1. A steep decrease in the expression level of G3.IL-2 was observed at the post-induction specific growth rates higher than its optimal value (0.056 h-1). In the induced recombinant cultures, plasmid multimerization was observed and highly dependent on specific growth and production rate: a higher post-induction specific growth rate and an increased specific production rate tended to significantly promote it much further. Moreover, plasmid stability was found to decrease rapidly in a faster growing culture.
Keyword
Recombinant human interleukin-2Specific production ratePlasmid multimerizationEscherichia coli
ISSN
0378-1097
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1111/j.1574-6968.1999.tb08751.x
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.