Transgenic tobacco plants expressing the bacterial levansucrase gene show enhanced tolerance to osmotic stress

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Title
Transgenic tobacco plants expressing the bacterial levansucrase gene show enhanced tolerance to osmotic stress
Author(s)
Jeong Mee ParkSuk Yoon Kwon; Ki Bang Song; Ju Won Kwak; Suk Bae Lee; Young Woo Nam; Jeong Sheop Shin; Young In Park; Sang Ki Rhee; Kyung Hee Paek
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 9, no. 2, pp. 213-218
Publication Year
1999
Abstract
Fructans are polyfructose molecules that function as nonstructural storage carbohydrates in several plants. In addition, it has been suggested that, due to their solubility, they can play an important role in helping plants survive periods of osmotic stress. In order to study the effect of levan synthesis on plant growth, the coding region of the levansucrase gene, which was isolated from Zymomonas mobilis, was introduced into tobacco plants using Agrobacterium tumefaciens-mediated transformation. The presence of the levansucrase gene in transgenic plants was verified by genomic DNA gel blot analysis. RNA gel blot and immunoblot analyses showed an accumulation of the corresponding transcript and protein product of the bacterial levansucrase gene in transgenic plants. Furthermore, a thin layer chromatography analysis revealed that fructans were synthesized and deposited in transgenic tobacco plants. When T1 seeds were germinated and grown under polyethylene glycol- mediated drought stress or cold stress, the transgenic seedlings displayed a substantially higher level of growth than that of untransformed plants. These results suggest that fructans may play a significant role in the tolerance of plants under osmotic stress.
Keyword
Levansucraseosmotic stresstobaccotransgenic plantZymomonas mobilis
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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