Nitric oxide synthase inhibitor decrease NMDA-induced elevations of extracellular glutamate and intracellular ca(2+) levels via a cGMP-independent mechanism in cerebellar granule neurons

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dc.contributor.authorSei Kwan Oh-
dc.contributor.authorBong Sik Yun-
dc.contributor.authorIn Ja Ryoo-
dc.contributor.authorPatrick P McCaslin-
dc.contributor.authorIck Dong Yoo-
dc.date.accessioned2017-04-19T08:56:29Z-
dc.date.available2017-04-19T08:56:29Z-
dc.date.issued1999-
dc.identifier.issn0253-6269-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4871-
dc.description.abstractThese studies were designed to examine the differential effect of nitric oxide (NO) and cGMP on glutamate neurotransmission. In primary cultures of rat cerebellar granule cells, the glutamate receptor agonist N-methyl-D-aspartate (NMDA) stimulates the elevation of intracellular calcium concentration ([Ca2+]i), the release of glutamate, the synthesis of NO and an increase of cGMP. Although NO has been shown to stimulate guanylyl cyclase, it is unclear yet whether NO alters the NMDA-induced glutamate release and [Ca2+]i elevation. We showed that the NO synthase inhibitor, NG-monomethyl-L-arginine (NMMA), partially prevented the NMDA-induced release of glutamate and elevation of [Ca2+]i and completely blocked the elevation of cGMP. These effects of NO on glutamate release and [Ca2+]i elevation were unlikely to be secondary to cGMP as the cGMP analogue, dibutyryl cGMP (dBcGMP), did not suppress the effects of NMDA. Rather, dBcGMP slightly augmented the NMDA-induced elevation of [Ca2+]i with no change in the basal level of glutamate or [Ca2+]i. The extracellular NO scavenger hydroxocobalamine prevented the NMDA-induced release of glutamate providing indirect evidence that the effect of NO may act on the NMDA receptor. These results suggest that low concentration of NO has a role in maintaining the NMDA receptor activation in a cGMP-independent manner.-
dc.publisherPharmaceutical Soc Korea-
dc.titleNitric oxide synthase inhibitor decrease NMDA-induced elevations of extracellular glutamate and intracellular ca(2+) levels via a cGMP-independent mechanism in cerebellar granule neurons-
dc.title.alternativeNitric oxide synthase inhibitor decrease NMDA-induced elevations of extracellular glutamate and intracellular ca(2+) levels via a cGMP-independent mechanism in cerebellar granule neurons-
dc.typeArticle-
dc.citation.titleArchives of Pharmacal Research-
dc.citation.number1-
dc.citation.endPage54-
dc.citation.startPage48-
dc.citation.volume22-
dc.contributor.affiliatedAuthorBong Sik Yun-
dc.contributor.affiliatedAuthorIn Ja Ryoo-
dc.contributor.affiliatedAuthorIck Dong Yoo-
dc.contributor.alternativeName오세관-
dc.contributor.alternativeName윤봉식-
dc.contributor.alternativeName류인자-
dc.contributor.alternativeNameMcCaslin-
dc.contributor.alternativeName유익동-
dc.identifier.bibliographicCitationArchives of Pharmacal Research, vol. 22, no. 1, pp. 48-54-
dc.identifier.doi10.1007/BF02976435-
dc.subject.keywordNMDA-
dc.subject.keywordNG-Monomethyl-L-arginine-
dc.subject.keywordDibutyryl-cGMP-
dc.subject.keywordCa2+ influx-
dc.subject.keywordHydroxocobalamin-
dc.subject.keywordGlutamate release-
dc.subject.localNMDA-
dc.subject.localNG-Monomethyl-L-arginine-
dc.subject.localDibutyryl-cGMP-
dc.subject.localCa2+ influx-
dc.subject.localHydroxocobalamin-
dc.subject.localGlutamate release-
dc.description.journalClassY-
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Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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