Isolation of a non-insecticidal Bacillus thuringiensis strain belonging to serotype H8a8b

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Title
Isolation of a non-insecticidal Bacillus thuringiensis strain belonging to serotype H8a8b
Author(s)
H W Park; J Y Roh; Y H Je; B R Jin; Hyun Woo Oh; H Y Park; S K Kang
Bibliographic Citation
Letters in Applied Microbiology, vol. 27, pp. 62-66
Publication Year
1998
Abstract
Bacillus thuringiensis strains non-toxic to Lepidoptera, Bombyx mori and Diptera, Culex pipiens pallens larvae were isolated from Korean soil samples during an investigation of B. thuringiensis isolates highly toxic to insect pests. One of these isolates, NTB-88, produces parasporal inclusions about 138 kDa in size and is non-toxic to 19 insect species of three orders, Lepidoptera, Diptera and Coleoptera, even though it is highly susceptible to tryptic cleavage. Study of flagellar (H) antibodies of 33 B. thuringiensis strains revealed that NTB-88 has an K antigen identical with that of subsp. morrisoni (serotype 8a8b). Comparison of parasporal inclusion proteins and plasmid DNA patterns of strain NTB-88 with B. thuringiensis subsp. morrisoni HD-12 and B. thuringiensis subsp. morrisoni PG-14 showed that the isolate is a novel non-insecticidal B. thuringiensis strain belonging to serotype 8a8b.
ISSN
0266-8254
Publisher
Wiley
DOI
http://dx.doi.org/10.1046/j.1472-765X.1998.00388.x
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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