Preliminary X-ray crystallographic analysis of a novel phytase from a Bacillus amyloliquefaciens

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dc.contributor.authorNam Chul Ha-
dc.contributor.authorYoung Ok Kim-
dc.contributor.authorTae Kwang Oh-
dc.contributor.authorByung Ha Oh-
dc.date.accessioned2017-04-19T08:56:33Z-
dc.date.available2017-04-19T08:56:33Z-
dc.date.issued1999-
dc.identifier.citationActa Crystallographica. Section D, Biological Crystallography,55,0,691,693ko
dc.identifier.issn0907-4449-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4891-
dc.description.abstractA novel bacterial phytase from a Bacillus amyloliquefaciens strain was crystallized using the hanging-drop vapour-diffusion method. The amino-acid sequence of the enzyme does not show any homology to those of other known phytases or phosphatases, with the exception of a phytase from Bacillus subtilis. The enzyme exhibits a thermal stability which is strongly dependent on calcium ions. High-quality single crystals of the enzyme in the absence of calcium ions were obtained using a precipitant solution containing 20% 2-methyl-2,4-pentanediol and 0.1 MMES (pH 6.5). Native diffraction data to 2.0 ? resolution were obtained from a flash-frozen crystal at 110 K using a rotating-anode X-ray source. The crystals belong to space group P212121 with unit-cell dimensions a = 50.4, b = 64.1, c = 104.2 ? and contain one monomer per asymmetric unit. Structure determination using heavy-atom derivative crystals is in progress, along with an effort to crystallize the calcium ion bound form of the enzyme.-
dc.publisherInt Union Crystallography-
dc.titlePreliminary X-ray crystallographic analysis of a novel phytase from a Bacillus amyloliquefaciens-
dc.title.alternativePreliminary X-ray crystallographic analysis of a novel phytase from a Bacillus amyloliquefaciens-
dc.typeArticle-
dc.citation.titleActa Crystallographica Section D-Biological Crystallography-
dc.citation.number0-
dc.citation.endPage693-
dc.citation.startPage691-
dc.citation.volume55-
dc.contributor.affiliatedAuthorTae Kwang Oh-
dc.contributor.alternativeName하남철-
dc.contributor.alternativeName김영옥-
dc.contributor.alternativeName오태광-
dc.contributor.alternativeName오병하-
dc.identifier.bibliographicCitationActa Crystallographica Section D-Biological Crystallography, vol. 55, pp. 691-693-
dc.identifier.doi10.1107/S0907444998015285-
dc.description.journalClassY-
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Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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