DC Field | Value | Language |
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dc.contributor.author | Nam Chul Ha | - |
dc.contributor.author | Young Ok Kim | - |
dc.contributor.author | Tae Kwang Oh | - |
dc.contributor.author | Byung Ha Oh | - |
dc.date.accessioned | 2017-04-19T08:56:33Z | - |
dc.date.available | 2017-04-19T08:56:33Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | Acta Crystallographica. Section D, Biological Crystallography,55,0,691,693 | ko |
dc.identifier.issn | 0907-4449 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4891 | - |
dc.description.abstract | A novel bacterial phytase from a Bacillus amyloliquefaciens strain was crystallized using the hanging-drop vapour-diffusion method. The amino-acid sequence of the enzyme does not show any homology to those of other known phytases or phosphatases, with the exception of a phytase from Bacillus subtilis. The enzyme exhibits a thermal stability which is strongly dependent on calcium ions. High-quality single crystals of the enzyme in the absence of calcium ions were obtained using a precipitant solution containing 20% 2-methyl-2,4-pentanediol and 0.1 MMES (pH 6.5). Native diffraction data to 2.0 ? resolution were obtained from a flash-frozen crystal at 110 K using a rotating-anode X-ray source. The crystals belong to space group P212121 with unit-cell dimensions a = 50.4, b = 64.1, c = 104.2 ? and contain one monomer per asymmetric unit. Structure determination using heavy-atom derivative crystals is in progress, along with an effort to crystallize the calcium ion bound form of the enzyme. | - |
dc.publisher | Int Union Crystallography | - |
dc.title | Preliminary X-ray crystallographic analysis of a novel phytase from a Bacillus amyloliquefaciens | - |
dc.title.alternative | Preliminary X-ray crystallographic analysis of a novel phytase from a Bacillus amyloliquefaciens | - |
dc.type | Article | - |
dc.citation.title | Acta Crystallographica Section D-Biological Crystallography | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 693 | - |
dc.citation.startPage | 691 | - |
dc.citation.volume | 55 | - |
dc.contributor.affiliatedAuthor | Tae Kwang Oh | - |
dc.contributor.alternativeName | 하남철 | - |
dc.contributor.alternativeName | 김영옥 | - |
dc.contributor.alternativeName | 오태광 | - |
dc.contributor.alternativeName | 오병하 | - |
dc.identifier.bibliographicCitation | Acta Crystallographica Section D-Biological Crystallography, vol. 55, pp. 691-693 | - |
dc.identifier.doi | 10.1107/S0907444998015285 | - |
dc.description.journalClass | Y | - |
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