Isolation of Not I Clusters Hypomethylated in HBV-Integrated Hepatocellular Carcinomas by Two-Dimensional Electrophoresis

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Title
Isolation of Not I Clusters Hypomethylated in HBV-Integrated Hepatocellular Carcinomas by Two-Dimensional Electrophoresis
Author(s)
Hisaki Nagai; Masaru Baba; Noboru Konishi; Yong Sung Kim; Masahiro Nogami; Katsuzumi Okumura; Mitsuru Emi; Ken-ichi Matsubara
Bibliographic Citation
DNA Research, vol. 6, pp. 219-225
Publication Year
1999
Abstract
To examine genetic and epigenetic alterations associated with HBV integration in hepatocarcinogenesis, we compared genomic DNA profiles of primary hepatocellular carcinomas (HCCs) and cell lines that either contained or did not contain integrated HBV. To accomplish this, we carried out Restriction Landmark Genomic Scanning (RLGS), a two-dimensional system that displays 2000-3000 Not I landmark sites in a single gel electrophoresis experiment. We identified one Not I landmark spot that showed high signal intensity in HBV-integrated cell lines or in primary HCCs, but not in HCCs or tumor-cell lines free of HBV integration. Cloning of this spot revealed that it consisted of a Not I cluster sequence enriched with CpG dinucleotides. This sequence, hypomethylated in association with HBV integration, was found in the peri-centromeric region of human acrochromosomes. The results demonstrate that epigenetic changes at specific sequences in the genome occur in association with HBV integration during the process of hepatocarcinogenesis. [The sequence data have been deposited in the EMBL database under accession number Y10751].
Keyword
Restriction Landmark Genomic Scanning (RLGS)hepatocellular carcinoma (HCC)hepatitis B Virus (HBV)methylation
ISSN
1340-2838
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1093/dnares/6.4.219
Type
Article
Appears in Collections:
Division of Biomedical Research > Genome Editing Research Center > 1. Journal Articles
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