Single-step purification of proteins of interest from proteolytically cleaved recombinant maltose-binding protein (MBP) fusion proteins by selective immunoprecipitation of MBP

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dc.contributor.authorJung Hyun Park-
dc.contributor.authorShin Young Na-
dc.contributor.authorDong Gun Lee-
dc.contributor.authorByoung Don Han-
dc.contributor.authorKil Lyong Kim-
dc.date.accessioned2017-04-19T08:56:35Z-
dc.date.available2017-04-19T08:56:35Z-
dc.date.issued1998-
dc.identifier.issn12268372-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4910-
dc.description.abstractThe maltose binding protein (MBP) fusion protein system is a versatile tool to express and isolate recombinant proteins in E. coli. In this system, MBP fusion proteins are efficiently isolated from whole cell lysate using amylose conjugated agarose beads and then eluted by competition with free maltose. Since MBP is a rather large molecule (∼42 kDa), for further experiments, the MBP part is usually proteolytically cleaved from the fusion protein and subsequently removed by ion-exchange chromatography or rebinding to amylose columns after washing out excess and MBP-bound maltose. In the present study, we have developed an improved method for the removal of cleaved MBP, which is advantageous over conventional methods. In this method, factor Xa cleaved MBP fusion proteins were incubated with Sepharose beads conjugated with MBP specific monoclonal antibodies and then precipitated by centrifugation, resulting in highly purified proteins in the supernatant.-
dc.publisherSouth Korea-
dc.titleSingle-step purification of proteins of interest from proteolytically cleaved recombinant maltose-binding protein (MBP) fusion proteins by selective immunoprecipitation of MBP-
dc.title.alternativeSingle-step purification of proteins of interest from proteolytically cleaved recombinant maltose-binding protein (MBP) fusion proteins by selective immunoprecipitation of MBP-
dc.typeArticle-
dc.citation.titleBiotechnology and Bioprocess Engineering-
dc.citation.number0-
dc.citation.endPage86-
dc.citation.startPage82-
dc.citation.volume3-
dc.contributor.alternativeName박정현-
dc.contributor.alternativeName나신영-
dc.contributor.alternativeName이동건-
dc.contributor.alternativeName한병돈-
dc.contributor.alternativeName김길룡-
dc.identifier.bibliographicCitationBiotechnology and Bioprocess Engineering, vol. 3, pp. 82-86-
dc.identifier.doi10.1007/BF02932507-
dc.subject.keywordmaltose binding protein (MBP)-
dc.subject.keywordfactor Xa-
dc.subject.keywordrecombinant protein-
dc.subject.keywordprotein purification-
dc.subject.keywordanti-MBP monoclonal antibody-
dc.subject.localmaltose binding protein (MBP)-
dc.subject.localMaltose binding protein-
dc.subject.localfactor Xa-
dc.subject.localFactor Xa-
dc.subject.localrecombinant protein-
dc.subject.localRecombinant protein-
dc.subject.localprotein purification-
dc.subject.localProtein purification-
dc.subject.localanti-MBP monoclonal antibody-
dc.description.journalClassY-
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