Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)

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Title
Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)
Author(s)
Dong Uk Kim; Tae Young Roh; Ji Eun Lee; Joo Yoon Noh; Young Joo Jang; Kwang Lae Hoe; Hyang Sook Yoo; Myung Un Choi
Bibliographic Citation
Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids, vol. 1437, no. 3, pp. 409-414
Publication Year
1999
Abstract
We cloned and expressed a full-length cDNA encoding a phospholipase D of type α (PLDα) from cabbage. Analysis of the cDNA predicted an 812-amino- acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD.
Keyword
Phospholipase DcDNA sequenceExpression(Brassica oleracea var. capitata)
ISSN
1388-1981
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S1388-1981(99)00020-7
Type
Article
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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