Open Access@KRIBBDivision of A.I. & Biomedical ResearchDigital Biotech Innovation Center1. Journal Articles
Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)
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- Title
- Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)
- Author(s)
- Dong Uk Kim; Tae Young Roh; Ji Eun Lee; Joo Yoon Noh; Young Joo Jang; Kwang Lae Hoe; Hyang Sook Yoo; Myung Un Choi
- Bibliographic Citation
- Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids, vol. 1437, no. 3, pp. 409-414
- Publication Year
- 1999
- Abstract
- We cloned and expressed a full-length cDNA encoding a phospholipase D of type α (PLDα) from cabbage. Analysis of the cDNA predicted an 812-amino- acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD.
- ISSN
- 1388-1981
- Publisher
- Elsevier
- Full Text Link
- http://dx.doi.org/10.1016/S1388-1981(99)00020-7
- Type
- Article
- Appears in Collections:
- Division of A.I. & Biomedical Research > Digital Biotech Innovation Center > 1. Journal Articles
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