Screening for L-sorbose and L-sorbosone dehydrogenase producing microbes for 2-keto-L-gulonic acid production

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Title
Screening for L-sorbose and L-sorbosone dehydrogenase producing microbes for 2-keto-L-gulonic acid production
Author(s)
Hong Weon Lee; Jae Gu Pan
Bibliographic Citation
Journal of Industrial Microbiology & Biotechnology, vol. 23, pp. 106-111
Publication Year
1999
Abstract
Acetic acid bacteria incompletely oxidize L-sorbose to 2-keto-L-gulonic acid (2KLG) by L-sorbose- and L-sorbosone dehydrogenases. In order to isolate novel microorganisms with these enzyme activities, a new screening method has been studied with a presumption that microorganisms reuse their metabolic products when principal carbon sources are exhausted. When various keto-aldonic acid-producing microorganisms were tested for the ability to grow in minimal media containing such products as 2,5-diketo-gluconic acid, 2-keto-D-gluconic acid, 5-keto-D-gluconic acid or 2-keto-L-gulonic acid, they grew with these keto-aldonic acids as the sole carbon source. By enriching the isolates collected from screening samples for their growth in minimal medium containing 2KLG as the sole carbon source, as much as 50% of selected strains showed L-sorbose- and L-sorbosone dehydrogenase activities. In spite of the presence of these enzymes, no significant amount of 2KLG was detected in the culture broth, possibly due to 2KLG reductase activity, indicating that the direct screening for 2KLG producer microorganisms would be less successful. These results suggest that the screening strategy using 2KLG as a carbon source is a useful method for the selective screening of microorganisms with L-sorbose- and L-sorbosone dehydrogenases, and that a similar strategy may be applied to other cases.
Keyword
L-sorbose dehydrogenaseL-sorbosone dehydrogenaseacetic acid bacteriascreening2-keto-L-gulonate
ISSN
0169-4146
Publisher
Springer
DOI
http://dx.doi.org/10.1038/sj.jim.2900697
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > 1. Journal Articles
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