Sequence analysis of 16S rRNA genes amplified from two ribosomal RNA gene clusters of Bifidobacterium bifidum

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Title
Sequence analysis of 16S rRNA genes amplified from two ribosomal RNA gene clusters of Bifidobacterium bifidum
Author(s)
Jung Hoon Yoon; Dong Koo Lim; Michael Goodfellow; Yong Ha Park
Bibliographic Citation
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, vol. 75, pp. 329-333
Publication Year
1999
Abstract
Two rRNA gene clusters were detected in the genome of Bifidobacterium bifidum KCTC 3202(T) using Southern blot analysis. To analyse the sequences of the 16S rRNA genes from rrnA and rrnB, 16S rDNAs were amplified by PCR using DNA fragments purified from gel slices containing each of the rRNA gene clusters. The amplified 16S rDNAs from rrnA and rrnB were cloned into vectors and three clones of each gene sequenced. The resultant sequences were confirmed by direct sequencing of the 16S rDNAs from rrnA and rrnB. Sequence differences were not found between rrnA and rrnB in 1488 bp of the 16S rRNA genes.
Keyword
Bifidobacterium bifidumrRNA operon16S rDNA
ISSN
0003-6072
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1002025100444
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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