Induction of cytotoxic T lymphocytes with peptides in vitro: identification of candidate T-cell epitopes in hepatitis B virus X antigen

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dc.contributor.authorMi Kyung Chung-
dc.contributor.authorHee Sik Yoon-
dc.contributor.authorSung Shik Min-
dc.contributor.authorHee Gu Lee-
dc.contributor.authorYoung Jae Kim-
dc.contributor.authorTae Gyu Lee-
dc.contributor.authorJong Soon Lim-
dc.contributor.authorChang Min Kim-
dc.contributor.authorSue Nie Park-
dc.date.accessioned2017-04-19T08:56:38Z-
dc.date.available2017-04-19T08:56:38Z-
dc.date.issued1999-
dc.identifier.issn1053-8550-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4927-
dc.description.abstractCytotoxic T lymphocytes (CTL) have been suggested to contribute to viral clearance during hepatitis B virus (HBV) infection. To induce effective CTL against vital infection by peptide vaccination, it is essential to identify the epitope peptides recognized by CTL. Here, 15 peptide sequences that contain HLA-A2.1-restricted CTL binding consensus motif were identified on hepatitis B virus X (HBx) protein and synthesized for further characterization. In the binding assay, 8 of 15 synthetic peptides enhanced the expression of HLA-A2.1 molecules on the surface of T2 cells, a human transport-associated antigen processing-deficient cell line. This result implies that these eight peptides are able to bind to the HLA-A2.1 molecules. These peptides were further tested for their ability to activate CTL from peripheral blood mononuclear cells (PBMCs) isolated from HBV chronic carriers. Five of eight tested peptides activated PBMC-derived T cells, resulting in the lysis of the target T2 cells pulsed with the same peptide. Furthermore, the CTL responses to HBx antigen in HBV chronic carriers were shown to be polyclonal, multispecific, and mediated mainly by CD8+ T cells. In contrast, these responses were not detected in uninfected healthy blood donors. Although the five CTL epitope peptides identified in this study have not been proven to be the naturally processed epitopes in HBV-infected hepatocytes, they could be candidates for peptide-based immunotherapy against HBV infection.-
dc.publisherKluwer-
dc.titleInduction of cytotoxic T lymphocytes with peptides in vitro: identification of candidate T-cell epitopes in hepatitis B virus X antigen-
dc.title.alternativeInduction of cytotoxic T lymphocytes with peptides in vitro: identification of candidate T-cell epitopes in hepatitis B virus X antigen-
dc.typeArticle-
dc.citation.titleJournal of Immunotherapy-
dc.citation.number4-
dc.citation.endPage287-
dc.citation.startPage279-
dc.citation.volume22-
dc.contributor.affiliatedAuthorHee Gu Lee-
dc.contributor.alternativeName정미경-
dc.contributor.alternativeNameYoon-
dc.contributor.alternativeNameMin-
dc.contributor.alternativeName이희구-
dc.contributor.alternativeNameKim-
dc.contributor.alternativeNameLee-
dc.contributor.alternativeNameLim-
dc.contributor.alternativeNameKim-
dc.contributor.alternativeName박순희-
dc.identifier.bibliographicCitationJournal of Immunotherapy, vol. 22, no. 4, pp. 279-287-
dc.identifier.doi10.1097/00002371-199907000-00001-
dc.subject.keywordCTL-
dc.subject.keywordEpitope peptide-
dc.subject.keywordHBx protein-
dc.subject.keywordHepatitis B virus-
dc.subject.keywordHLA-A2.1-
dc.subject.localCTL-
dc.subject.localEpitope peptide-
dc.subject.localHBx protein-
dc.subject.localhepatitis B virus (HBV)-
dc.subject.localHepatitis B Virus-
dc.subject.localHepatitis B virus (HBV)-
dc.subject.localhepatitis B virus-
dc.subject.localhepatitis B Virus (HBV)-
dc.subject.localHepatitis B virus-
dc.subject.localHLA-A2.1-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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