Prevention of virus-induced diabetes by single immunization with recombinant BCG in SJL/J male mice = 제조합 BCG의 일회 면역에 의한 쥐에서 바이러스성 당뇨병 예방

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Title
Prevention of virus-induced diabetes by single immunization with recombinant BCG in SJL/J male mice = 제조합 BCG의 일회 면역에 의한 쥐에서 바이러스성 당뇨병 예방
Author(s)
Sang Hyun Cho; Beom Kyu Choi; Gill Han Bai; Sang Jae Kim; Byung Hwa Hyun; Yong Kyung Choe; Hyung Hoan Lee; Yong Soo Bae
Bibliographic Citation
Korean Journal of Immunology, vol. 21, no. 3, pp. 259-269
Publication Year
1999
Abstract
The D variant of encephalomyocarditis (EMC-D) virus causes diabetes in susceptible mice by direct cytolysis of pancreatic beta-cells. cDNA covering the major outer capsid protein (VP1) of the EMC-D virus was cloned into Mycobacterium bovis bacillus Calmette-Guerin (BCG). None of the SJL/J mice immunized with live recombinant BCG-VP1 (rBCG-VP1) became diabetic when challenged with the highly diabetogenic EMC-D virus, but the control mice inoculated with normal BCG developed diabetes during the same challenge. VP1-specific antibodies (including neutralizing antibodies) were markedly increased over time and reached the maximum titer at week 10 after a single immunization. The plateau of the titer lasted longer than 4 weeks. Mice and guinea pigs immunized with live rBCG-VP1 showed strong delayed-type hypersensitivity to the VP1 of the EMC-D virus. The preventive immunity still worked effectively 10 months after the primary immunization. At that time, the VP1-specific antibody was almost undetectable in the bloodstream, but a large number of VP1-specific lymphocytes was found in the spleen of the immunized mice. Our results show that live rBCG-VP1 elicits effective humoral and long-lasting cellular immune responses against EMC-D virus infection that results in the prevention of virus-induced diabetes in susceptible mice.
Keyword
recombinant BCGvirus-induced diabetesprevention
ISSN
I000-0015
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.2337/diabetes.49.9.1459
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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