Screening of mushrooms having fibrinolytic activity = 혈전용해능을 갖는 버섯류의 탐색

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Title
Screening of mushrooms having fibrinolytic activity = 혈전용해능을 갖는 버섯류의 탐색
Author(s)
Nack Shick Choi; Sung Yum Seo; Seung Ho Kim
Bibliographic Citation
Korean Journal of Food Science & Technology, vol. 31, no. 2, pp. 553-557
Publication Year
1999
Abstract
Five fungi (mushrooms), Daedaleopsis styracina, Trichaptum abietium, Coriolus versicolor, Pisolithus tinctorius and Tricholomopsis decora, were screened and examined the fibrinolytic activity and specificity. The extracts of mushrooms showed a level of fibrinolytic activity that was about 3-4 times higher than that of plasmin 1.0 unit. In particular, Pisolithus tinctorius of them showed the greatest enzyme activity (4.71 plasmin unit/mL) by fibrin plate assay, and the highest specificity (1.32 plasmin unit/mL) using chromogenic substrate (N-p-Tosyl-Gly-Pro-Lys p-nitroanilide) by Tricholomopsis decora. And the same molecular mass 54 and 61kDa showing the fibrinolytic activity obtained from all fruiting bodies were confirmed, and it was found that Trichaptum abietium and Tricholomopsis decora have a strong fibrinolytic enzyme with an apparent size of 100 kDa and 84 kDa, respectively on SDS-fibrin zymography activity assay.
Keyword
mushroomfibrinolytic enzymefibrin zymography
ISSN
0367-6293
Publisher
South Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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