Optimization of immunosasay procedures for the measurement of progesterone = Progesterone 측정을 위한 면역분석법의 최적조건에 관한 연구

Abstract

This research was carried out to investigate the optimal conditions associated with the RIA procedures such as a bridging phenomena, prozone effects and a new separition methods etc. The results obtained were summarized as follows: 1. The IgG fractions of donkey anti-rabbit IgG sera were purified by Protein-A-Sepharose affinity column, which indicates that Protein-A an affinity for IgG class of donkey antiserum. 2. In coating the IgG fraction on polystryrene tubes, incubation conditions made ni differences between 2 hr at room remperature and overnight at 4˚C. 3. There were no significant differences between 1st antibody-coated tube and 2nd antibody-coated tube as a separation method when compared in terms of reproducbility. A better reproducibility may be expected of the titers of 1st antibody for the progesterone to be assayed and of corresponding 2nd antibody are reasonably high.