In vitro activation of yeast pro-carboxypeptidase Y expressed as inclusion bodies in Escherichia coli

Cited 7 time in scopus
Metadata Downloads
Title
In vitro activation of yeast pro-carboxypeptidase Y expressed as inclusion bodies in Escherichia coli
Author(s)
Moon Sun Hahm; Jung Hoon Bae; Eui Sung Choi; Bong Hyun Chung
Bibliographic Citation
Biotechnology Letters, vol. 21, pp. 881-885
Publication Year
1999
Abstract
The genes encoding pro-carboxypeptidase Ys (pro-CPYs) from Saccharomyces cerevisiae and Hansenula polymorpha were cloned and expressed in Escherichia coli. Most of the expressed pro-CPY was present in the form of inclusion bodies, accounting for about 35% of the total cellular protein. The inclusion bodies solubilized in 6 M guanidinum chloride were renatured by dilution 1:60 into renaturation buffer. Further refolding and activation were followed by the addition of 60 μg ml-1 proteinase K into the diluted solution. The specific activities of in vitro activated S. cerevisiae and H. polymorpha CPYs were found to be similar, representing about 25% of that of native S. cerevisiae CPY.
Keyword
E. coliH. polymorphain vitro activationpro-carboxypeptidase Yproteinase K
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1005506119165
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.