Overexpression of cyclodextrin glycosyltransferase gene from Brevibacillus brevis in Escherichia coli by control of temperature and mannitol concentration

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dc.contributor.authorMyung Hee Kim-
dc.contributor.authorJung Kee Lee-
dc.contributor.authorHyung Kwoun Kim-
dc.contributor.authorCheon Bae Sohn-
dc.contributor.authorTae Kwang Oh-
dc.date.accessioned2017-04-19T08:56:42Z-
dc.date.available2017-04-19T08:56:42Z-
dc.date.issued1999-
dc.identifier.issn0951-208X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4957-
dc.description.abstractExpression of Brevibacillus brevis CD 162 cyclodextrin glycosyltransferase (CGTase) gene using pET22b(+) vector in Escherichia coli BL21 (DE3) resulted in the formation of inactive inclusion bodies under the usual induction conditions. However, by lowering the induction temperature to 30°C and/or adding 0.5 M mannitol as an osmolyte, the formation of insoluble aggregates was prevented and about a 34-fold increase (8.51 U ml-1) in biologically active soluble form was achieved after 6 h induction. The active CGTase enzyme was estimated to comprise as much as 24% of the total soluble proteins. In addition, other polyols such as glycerol, erythritol, xylitol, sorbitol, and arabitol showed similar effects with mannitol on the production of active CGTase enzyme.-
dc.publisherSpringerko
dc.titleOverexpression of cyclodextrin glycosyltransferase gene from Brevibacillus brevis in Escherichia coli by control of temperature and mannitol concentration-
dc.title.alternativeOverexpression of cyclodextrin glycosyltransferase gene from Brevibacillus brevis in Escherichia coli by control of temperature and mannitol concentration-
dc.typeArticle-
dc.citation.titleBiotechnology Techniques-
dc.citation.number0-
dc.citation.endPage770-
dc.citation.startPage765-
dc.citation.volume13-
dc.contributor.affiliatedAuthorMyung Hee Kim-
dc.contributor.affiliatedAuthorJung Kee Lee-
dc.contributor.affiliatedAuthorHyung Kwoun Kim-
dc.contributor.affiliatedAuthorTae Kwang Oh-
dc.contributor.alternativeName김명희-
dc.contributor.alternativeName이정기-
dc.contributor.alternativeName김형권-
dc.contributor.alternativeName손천배-
dc.contributor.alternativeName오태광-
dc.identifier.bibliographicCitationBiotechnology Techniques, vol. 13, pp. 765-770-
dc.identifier.doi10.1023/A:1008909105229-
dc.subject.keywordBrevibacillus-
dc.subject.keywordcyclodextrin-
dc.subject.keywordgene expression-
dc.subject.keywordglycosyltransferase-
dc.subject.keywordmannitol-
dc.subject.keywordpolyol-
dc.subject.localBrevibacillus-
dc.subject.localCyclodextrin-
dc.subject.localcyclodextrin-
dc.subject.localGene Expression-
dc.subject.localGene expression-
dc.subject.localgene expression-
dc.subject.localGlycosyltransferase-
dc.subject.localglycosyltransferase-
dc.subject.localGlycosyltransferases-
dc.subject.localmannitol-
dc.subject.localpolyol-
dc.description.journalClassN-
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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