Purification and properties of inulaseII from arthrobacter ureafaciens KCTC 3387 = Arthrobacter ureafaciens KCTC 3387이 생산하는 inulase II의 정제 및 특성
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- Title
- Purification and properties of inulaseII from arthrobacter ureafaciens KCTC 3387 = Arthrobacter ureafaciens KCTC 3387이 생산하는 inulase II의 정제 및 특성
- Author(s)
- Jae Chan Lee; Ki Young Lee; Ki Bang Song; Yong Bok Lee
- Bibliographic Citation
- Korean Journal of (Applied) Microbiology & Biotechnology, vol. 27, no. 6, pp. 471-476
- Publication Year
- 1999
- Abstract
- Inulin fructotransferase(depolymerizing)(EC 2.4.1.93)(inulaseII) which converts inulin into di-D-fructofuranose-1,2':2,3'-dianhydride (DFAIII) was purified from Arthrobacter ureafaciens KCTC 3387 using column chromatography on DEAE-Toyopearl 650 M and gel filtration on Sephadex G-200. The enzyme was purified 7-fold with a yield of 11% from a culture supernatant. The purified enzyme gave a single band on polyacrylamide gel electrophoresis, and the molecular weight of the enzyme was estimated to be 45,000 by SDS- polyacrylamide gel electrophoresis. The optimum pH and temperature for the enzyme reaction were pH 6.5-7.0 and 55°C, respectively. The enzyme was stable within a pH range of 5.0 to 10.6 and up to 60°C. The K(m) of this enzyme for DFAIII production was 11.9 mM. The enzyme was inactivated by Hg2+ and after exhaustive digestion of inulin by this enzyme, 1-kestose and nystose were produced in addition to DFAIII.
- Keyword
- Arthrobacter ureafaciens KCTC 3387DFAIIIInulaseIIInulin
- ISSN
- 0257-2389
- Publisher
- Korea Soc-Assoc-Inst
- Type
- Article
- Appears in Collections:
- 1. Journal Articles > Journal Articles
- Files in This Item:
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