Extracellular secretion of levansucrase from Zymomonas mobilis in Escherichia coli

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dc.contributor.authorK H Jang-
dc.contributor.authorJeong Woo Seo-
dc.contributor.authorKi Bang Song-
dc.contributor.authorChul Ho Kim-
dc.contributor.authorSang Ki Rhee-
dc.date.accessioned2017-04-19T08:56:44Z-
dc.date.available2017-04-19T08:56:44Z-
dc.date.issued1999-
dc.identifier.issn0178-515X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4971-
dc.description.abstractSecretion of levansucrase from Zymomonas mobilis in Escherichia coli by glycine supplement was investigated. A significant amount of levansucrase (about 25% of total activity) was found in intact whole-cells. Cell fractionation experiments showed that levansucrase was found both in the periplasmic space and in the cytoplasmic fraction of E. coli. None or only trace amounts of levansucrase was detected in the extracellular culture broth at 24 h of cultivation and it accrued with the increasing concentration of glycine in the culture medium and duration of the culture period. Optimal glycine concentration for the maximum secretion of levansucrase was in the range of 0.8-1%, in which approximately 20-50% of levansucrase was released into the extracellular fraction at 24 h of cultivation, although glycine retarded the bacterial growth.-
dc.publisherSpringer-
dc.titleExtracellular secretion of levansucrase from Zymomonas mobilis in Escherichia coli-
dc.title.alternativeExtracellular secretion of levansucrase from Zymomonas mobilis in Escherichia coli-
dc.typeArticle-
dc.citation.titleBioprocess Engineering-
dc.citation.number5-
dc.citation.endPage458-
dc.citation.startPage453-
dc.citation.volume21-
dc.contributor.affiliatedAuthorJeong Woo Seo-
dc.contributor.affiliatedAuthorKi Bang Song-
dc.contributor.affiliatedAuthorChul Ho Kim-
dc.contributor.affiliatedAuthorSang Ki Rhee-
dc.contributor.alternativeName장기효-
dc.contributor.alternativeName서정우-
dc.contributor.alternativeName송기방-
dc.contributor.alternativeName김철호-
dc.contributor.alternativeName이상기-
dc.identifier.bibliographicCitationBioprocess Engineering, vol. 21, no. 5, pp. 453-458-
dc.identifier.doi10.1007/s004490050701-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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