Isolation of a high-yield mutant strain for L-proline production and its fermentation conditions

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Title
Isolation of a high-yield mutant strain for L-proline production and its fermentation conditions
Author(s)
Wuk Sang Ryu; Hyung Wook Jang; Kyoung Hee Cho; Soon Jae Chang; Yeon Woo Ryu; Young Hoon Park
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 9, no. 5, pp. 613-618
Publication Year
1999
Abstract
L-Proline-producing mutant strains were developed by exposing L-glutamic acid-producing bacteria to N-metyl-N-nitro-nitrosoguanidine and UV irradiation. A L-histidine auxotroph of Corynebacterium acetoacidophilum RYU3161 (KCTC 0616BP), which was resistant to sulfaguanidine and proline analogs (DHP, AZC, TAC), was isolated. The activity of the mutant strain's γ-glutamyl kinase was 45% higher than that of the parent strain. The optimum level of L-histidine for production of L-proline was 0.16 g/l. In a 5-1 jar fermenter, the mutant strain produced L-proline at a high concentration (35 g/l) level within 48 h of cultivation.
Keyword
L-prolineL-histidine auxotrophsulfaguanidine resistant mutantproline analog resistant mutantγ-glutamyl kinase
ISSN
1017-7825
Publisher
South Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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