Optimization of medium components for phytase production by E. coli using response surface methodology

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Title
Optimization of medium components for phytase production by E. coli using response surface methodology
Author(s)
K Sunitha; Jung Kee Lee; Tae Kwang Oh
Bibliographic Citation
Bioprocess Engineering, vol. 21, pp. 477-481
Publication Year
1999
Abstract
The medium for recombinant phytase production by E. coli BL21 was optimized using response surface methodology. A 23 central composite experimental design was used to study the combined effect of the medium components, tryptone, yeast extract and NaCl. Addition of 2 g/l glucose to the medium greatly influenced the phytase production. The optimization of the medium has increased the phytase production by 1.2 times. The incorporation of 2 g/l glucose significantly enhanced the phytase production by 1.58 times, showing an overall 2.78 fold increase before optimization and other modifications of the medium. The optimized medium with glucose showed a highest phytase activity of 2250 U/l.
ISSN
0178-515X
Publisher
Springer
DOI
http://dx.doi.org/10.1007/PL00009086
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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