High-level expression of a recombinant thermostable phytase in Bacillus subtilis

Cited 25 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorYoung Ok Kim-
dc.contributor.authorJung Kee Lee-
dc.contributor.authorByung Chul Oh-
dc.contributor.authorTae Kwang Oh-
dc.date.accessioned2017-04-19T08:56:46Z-
dc.date.available2017-04-19T08:56:46Z-
dc.date.issued1999-
dc.identifier.issn0916-8451-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4982-
dc.description.abstractAn efficient expression system was developed in Bacillus subtilis for the large scale production of phytase. The phytase gene with a native promoter derived from Bacillus amyloliquefaciens was cloned in the Bacillus expression vector pJH27 under a strong BJ27 promoter and its expression was optimized. The expression of the phytase gene occurred during late exponential growth and the extracellular phytase production was 2.0 units/ml, which constituted over 90% of the total protein. The yield was 100-fold higher than wild type Bacillus amyloliquefaciens DS11.-
dc.publisherT&F (Taylor & Francis)-
dc.titleHigh-level expression of a recombinant thermostable phytase in Bacillus subtilis-
dc.title.alternativeHigh-level expression of a recombinant thermostable phytase in Bacillus subtilis-
dc.typeArticle-
dc.citation.titleBioscience Biotechnology and Biochemistry-
dc.citation.number12-
dc.citation.endPage2207-
dc.citation.startPage2205-
dc.citation.volume63-
dc.contributor.affiliatedAuthorJung Kee Lee-
dc.contributor.affiliatedAuthorByung Chul Oh-
dc.contributor.affiliatedAuthorTae Kwang Oh-
dc.contributor.alternativeName김영옥-
dc.contributor.alternativeName이정기-
dc.contributor.alternativeName오병철-
dc.contributor.alternativeName오태광-
dc.identifier.bibliographicCitationBioscience Biotechnology and Biochemistry, vol. 63, no. 12, pp. 2205-2207-
dc.identifier.doi10.1271/bbb.63.2205-
dc.subject.keywordBacillus subtilis-
dc.subject.keywordphytase-
dc.subject.keywordphytate-
dc.subject.keywordoverexpression-
dc.subject.localBacillus subtilis-
dc.subject.localbacillus subtilis-
dc.subject.localphytase-
dc.subject.localPhytase-
dc.subject.localPhytate-
dc.subject.localphytate-
dc.subject.localOverexpression-
dc.subject.localoverexpression-
dc.subject.localover-expression-
dc.subject.localOver-expression-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.