Antifungal mechanism of a cysteine-rich antimicrobial peptide, Ib-AMP1, from Impatiens balsamina against Candida albicans

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Title
Antifungal mechanism of a cysteine-rich antimicrobial peptide, Ib-AMP1, from Impatiens balsamina against Candida albicans
Author(s)
Dong Gun Lee; Song Yup Shin; Dae Hee Kim; Moo Yeol Seo; Joo Hyun Kang; Young Hoon Lee; Kil Lyong Kim; Kyung Soo Hahm
Bibliographic Citation
Biotechnology Letters, vol. 21, no. 12, pp. 1047-1050
Publication Year
1999
Abstract
The antifungal mechanism of a 20-mer peptide, Ib-AMP1, derived from Impatiens balsamina was investigated. The oxidized (disulfide bridged) Ib- AMP1 showed a 4-fold increase in antifungal activity against Aspergillus flavus and Candida albicans than reduced (non-disulfide bridged) Ib-AMP1. Ib- AMP1 had very low activity for phospholipid disruption when compared with cecropin A(1-8)-magainin 2(1-12), a α-helical amphiphatic, antimicrobial peptide. Confocal microscopy showed that Ib-AMP1 binds on cell surface or penetrates into cell membranes. These results suggested that Ib-AMP1 may manifest its antifungal activity against Candida albicans by inhibiting a distinct cellular process rather than ion channel or pore formation in cell membrane.
Keyword
antifungal mechanismCandida albicansconfocal microscopyIb-AMP1
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1005636610512
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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