Characterization of lactoferrin gene 5' Flanking region of Korean native goat = 재래산양 락토페린 유전자 5' Flanking 영역의 특성에 관한 연구

Abstract

Lactoferrin(Lf), a ferric ion(Fe3+)-binding glycoprotein, is found most notably in milk and some exocrine secretions. It is a main antimicrobial component of colostrum and milk that contributes to protection of the infant against infectious diseases. To analyze 5’flanking region of Korean native goat lactoferrin gene, we cloned and sequenced 975 bp of 5’flanking region of Korean native goat Lf gene. When compared to those of bovine and human Lf, the nucleotide sequences exhibited about 90.9% and 49.7% homology, respectively. The transcription start site was localized by primer extension to residue C, which is 38 nucleotides upstream from the ATG start codon. The 5’flanking region of Korean native goat lactoferrin gene contains putative transcription factor binding sites such as SP1, CAT, C/EBP and GATA-1.