Isolation of defense-related genes from Nicotiana glutinosa infected by tobacco mosaic virus using a modified differential screening

Cited 0 time in scopus
Metadata Downloads
Title
Isolation of defense-related genes from Nicotiana glutinosa infected by tobacco mosaic virus using a modified differential screening
Author(s)
Kyung Soon Park; Mi Chung Suh; Jong Joo Cheong; Do Il Choi
Bibliographic Citation
Plant Pathology Journal, vol. 15, no. 5, pp. 295-301
Publication Year
1999
Abstract
Many of plant defense responses are consequence of transcriptional activation of related genes. We have developed a modified differential screening procedure to isolate tobacco genes that are involved in the defense responses against TMV infection. A cDNA library was constructed from Nicotiana glutinosa leaves infected by TMV under temperature shift conditions. Each of plasmid DNA in the library was hybridized on a set of slot blots to a pool of cDNA probes prepared from either TMV-infected or mock-treated tobacco leaves. Among 900 plasmid DNAs, 81 clones exhibiting significantly enhanced or reduced level of hybridization to either probe were selected for nucleotide sequencing. The clones were listed into 61 genes considering redundancy between the sequences. The genes were identified to be defense-related genes including PR-genes and genes involved in primary or secondary metabolisms. This results supports the implication that plant defense process entails a major shift in total cellular metabolisms rather than activation of a limited number of defense-related genes. Expression patterns of a number of defense-related genes. Expression patterns of a number of selected genes were examined in northern blot analyses. It is notable that the clone 630 of unknown function exhibits expression pattern similar to those of previously known PR-genes. Experiments to elucidate the roles in defense mechanism of a couple of genes newly identified in this study are in progress.
Keyword
defense-related genesdifferential screeningnicotiana tabacumplant defensetobacco mosaic virus
ISSN
I000-0113
Publisher
South Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.