Identification and characterization of an oil-degrading yeast, Yarrowia lipolytica 180

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Identification and characterization of an oil-degrading yeast, Yarrowia lipolytica 180
Tae Hyun Kim; Jung Hyun Lee; Young Sook Oh; Kyung Sook Bae; Sang Jin Kim
Bibliographic Citation
Journal of Microbiology, vol. 37, no. 3, pp. 128-135
Publication Year
Among oil-degrading microorganisms isolated from oil-polluted industrial areas, one yeast strain showed high degradation activity of aliphatic hydrocarbons. From the analyses of 18S rRNA sequences, fatty acid, coenzyme Q system, G+C content of DNA, and biochemical characteristics, the strain was identified as Yarrowia lipolytica 180. Y. lipolytica 180 degraded 94% of aliphatic hydrocarbons in minimal salts medium containing 0.2% (v/v) of Arabian light crude oil within 3 days at 25°C. Optimal growth conditions for temperature, pH, NaCl concentration, and crude oil concentration were 30°C, pH 5-7, 1%, and 2% (v/v), respectively. Y. lipolytica 180 reduced surface tension when cultured on hydrocarbon substrates (1%, v/v), and the measured values of the surface tension were in the range of 51 to 57 dynes/cm. Both the cell free culture broth and cell debris of Y. lipolytica 180 were capable of emulsifying 2% (v/v) crude oil by itself. They were also capable of degrading crude oil (2%). The strain showed a cell surface hydrophobicity higher than 90%, which did not require hydrocarbon substrates for its induction. These results suggest that Y. lipolytica has high oil-degrading activity through its high emulsifying activity and cell hydrophobicity, and further indicate that the cell surface is responsible for the metabolism of aliphatic hydrocarbons.
hydrocarbon degradationcell hydrophobicityYarrowia lipolytica 180bioemulsification
Microbiological Society Korea
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