Glucosamine-mediated detoxification of p-benzoquinone and its removal by chitosan

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Title
Glucosamine-mediated detoxification of p-benzoquinone and its removal by chitosan
Author(s)
Sang Ho Park; Oh Sung Kwon; Chang Won Choi; Chang Jin Kim
Bibliographic Citation
Biotechnology Letters, vol. 22, no. 1, pp. 21-24
Publication Year
2000
Abstract
p-Benzoquinone non-enzymatically reacted with D-glucosamine at physiological pH and moderate temperature. The reaction of p benzoquinone with glucosamine was signaled by changes in the UV and visible spectra. The reactivity proceeded fastest at pH values above 7, with a sharp drop from pH 6.5 to 7.0, and the reaction was negligible in acidic conditions. The order of reactivity of amino sugars was D-mannosamine > D-glucosamine > D- galactosamine. From the reaction mixture, four conversion products were isolated and none was toxic to Escherichia coli even at 500-700 μg ml-1, while p-benzoquinone was cytotoxic to E. coli at 20 μg ml-1. Chitosan could react with p-benzoquinone efficiently and remove this toxicant in aqueous solution.
Keyword
chitosandetoxificationp-benzoquinoneremoval
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1005648121726
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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