Molecular cloning of a cDNA encoding ribosome inactivating protein from Amaranthus viridis and its expression in E. coli

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dc.contributor.authorSuk Yoon Kwon-
dc.contributor.authorChung Sun An-
dc.contributor.authorJang Ryol Liu-
dc.contributor.authorSang Soo Kwak-
dc.contributor.authorHeang Soon Lee-
dc.contributor.authorJeong Kook Kim-
dc.contributor.authorKyung Hee Paek-
dc.date.accessioned2017-04-19T08:56:51Z-
dc.date.available2017-04-19T08:56:51Z-
dc.date.issued2000-
dc.identifier.issn1016-8478-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5006-
dc.description.abstractIn order to isolate a cDNA clone of ribosome inactivating protein (RIP), a cDNA library was constructed in Uni-ZAP XL vector with poly(A) RNA purified from leaves of Amaranthus viridis. To get the probe for screening the library, PCR of phage DNA was conducted using the vector primer and degenerate primer designed from a conserved putative active site of the RIPs. Twenty-six cDNA clones from about 600,000 plaques were isolated, and one of these clones was fully sequenced. It was 1,047 bp and contained an open reading frame encoding 270 amino acids. The deduced amino acid sequence had a putative signal sequence of 17 amino acids and a putative active site (AIQMVAEAARFFKYIE) conserved in other RIPs. E. coli cells expressing A. viridis RIP cDNA did not grow well as compared to control cells, indicating that recombinant A. viridis RIP presumably inactivated E. coli ribosomes. In addition, recombinant A. viridis RIP cDNA produced by E. coli had translation inhibition activity in vitro.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleMolecular cloning of a cDNA encoding ribosome inactivating protein from Amaranthus viridis and its expression in E. coli-
dc.title.alternativeMolecular cloning of a cDNA encoding ribosome inactivating protein from Amaranthus viridis and its expression in E. coli-
dc.typeArticle-
dc.citation.titleMolecules and Cells-
dc.citation.number1-
dc.citation.endPage12-
dc.citation.startPage8-
dc.citation.volume10-
dc.contributor.affiliatedAuthorSuk Yoon Kwon-
dc.contributor.affiliatedAuthorJang Ryol Liu-
dc.contributor.affiliatedAuthorSang Soo Kwak-
dc.contributor.affiliatedAuthorHeang Soon Lee-
dc.contributor.alternativeName권석윤-
dc.contributor.alternativeName안충순-
dc.contributor.alternativeName유장렬-
dc.contributor.alternativeName곽상수-
dc.contributor.alternativeName이행순-
dc.contributor.alternativeName김정국-
dc.contributor.alternativeName백경희-
dc.identifier.bibliographicCitationMolecules and Cells, vol. 10, no. 1, pp. 8-12-
dc.identifier.doi10.1007/s10059-000-0008-6-
dc.subject.keywordamaranthus viridis-
dc.subject.keywordcDNA-
dc.subject.keywordexpression-
dc.subject.keywordribosome inactivating protein-
dc.subject.localamaranthus viridis-
dc.subject.localAmaranthus viridis-
dc.subject.localcDNA-
dc.subject.localCdna-
dc.subject.localexpression-
dc.subject.localExpression-
dc.subject.localribosome inactivating protein-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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