Differential resistance to methyl viologen in transgenic tobacco plants that express sweet potato peroxidases

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Title
Differential resistance to methyl viologen in transgenic tobacco plants that express sweet potato peroxidases
Author(s)
Byung Wook Yun; Gyung Hye Huh; Heang Soon Lee; Suk Yoon Kwon; Jin Ki Jo; Jin Seog Kim; Kwang Yun Cho; Sang Soo Kwak
Bibliographic Citation
Journal of Plant Physiology, vol. 156, no. 4, pp. 504-509
Publication Year
2000
Abstract
To analyze the physiological role of each peroxidase (POD) isoenzyme in environmental stress adaptation, transgenic tobacco (Nicotiana tabacum) plants expressing either a sweet potato (Ipomoea batatas) anionic POD (swpa1) or neutral POD (swpn1) were tested for responses to oxidant methyl viologen (MV). Fully expanded leaves of both swpa1- and swpn1-transgenic plants had about two times higher POD activity than non transformed (NT) plants. When tobacco leaf discs were subjected to MV at 10 and 100 μmol/L, swpa1-transgenic plants showed about a 25 % reduction in membrane damage relative to swpn1-transgenic or NT plants. Leaves of swpn1-transgenic and NT plants were bleached more than those of swpa1-transgenics by 1 μmol/L MV treatment, whereas all plants were severely damaged at 3 μmol/L MV. These results indicate that the increased H2O2-scavenging capacity provided by the swpa1 POD (a guaiacol-type POD) contributes to increased protection against MV-mediated oxidative damage. Furthermore, differences in response to oxidant treatments indicate unique functions for the two isoenzymes encoded by swpa1 and swpn1.
Keyword
ipomoea batatasnicotiana tabacumsweet potatoion conductivitymethyl viologenoxidative stressperoxidase
ISSN
0176-1617
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S0176-1617(00)80165-0
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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