Differential resistance to methyl viologen in transgenic tobacco plants that express sweet potato peroxidases

Abstract

To analyze the physiological role of each peroxidase (POD) isoenzyme in environmental stress adaptation, transgenic tobacco (Nicotiana tabacum) plants expressing either a sweet potato (Ipomoea batatas) anionic POD (swpa1) or neutral POD (swpn1) were tested for responses to oxidant methyl viologen (MV). Fully expanded leaves of both swpa1- and swpn1-transgenic plants had about two times higher POD activity than non transformed (NT) plants. When tobacco leaf discs were subjected to MV at 10 and 100 μmol/L, swpa1-transgenic plants showed about a 25 % reduction in membrane damage relative to swpn1-transgenic or NT plants. Leaves of swpn1-transgenic and NT plants were bleached more than those of swpa1-transgenics by 1 μmol/L MV treatment, whereas all plants were severely damaged at 3 μmol/L MV. These results indicate that the increased H2O2-scavenging capacity provided by the swpa1 POD (a guaiacol-type POD) contributes to increased protection against MV-mediated oxidative damage. Furthermore, differences in response to oxidant treatments indicate unique functions for the two isoenzymes encoded by swpa1 and swpn1.