High frequency shoot induction and plant regeneration from cotyledonary hypocotyl explants of Cucumber(Cucumis sativus L.) seedlings

Cited 9 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJae Whune Kim-
dc.contributor.authorSu Kyung Han-
dc.contributor.authorSuk Yoon Kwon-
dc.contributor.authorHeang Soon Lee-
dc.contributor.authorYong Pyo Lim-
dc.contributor.authorJang Ryol Liu-
dc.contributor.authorSang Soo Kwak-
dc.date.accessioned2017-04-19T08:56:52Z-
dc.date.available2017-04-19T08:56:52Z-
dc.date.issued2000-
dc.identifier.issn0176-1617-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5009-
dc.description.abstractA method of high frequency shoot induction was established using excised hypocotyl segments bearing cotyledons (referred to as cotyledonary hypocotyl explants) of cucumber (Cucumis sativus L.) seedlings. Adventitious shoots were obtained from the upper excised hypocotyls of cucumber seedlings incubated on Murashige and Skoog (MS) medium containing 2.0 mg/L zeatin. The effects of cotyledon size and hypocotyl length of seedlings on shoot organogenesis were also examined. A higher frequency (80-90 %) of adventitious shoots was obtained from explants of 3- and 5-day-old seedlings with either 1 cotyledon or 2 half-cotyledons, and a short hypocotyl length (2 mm). However, explants with long hypocotyls and 2 cotyledons produced fewer shoots. Adventitious shoots from cotyledonary hypocotyl explants were removed and rooted in MS medium containing 1.0 mg/L IAA. The rooted plantlets developed into normal plants after acclimatization to ambient humidity levels.-
dc.publisherElsevier-
dc.titleHigh frequency shoot induction and plant regeneration from cotyledonary hypocotyl explants of Cucumber(Cucumis sativus L.) seedlings-
dc.title.alternativeHigh frequency shoot induction and plant regeneration from cotyledonary hypocotyl explants of Cucumber(Cucumis sativus L.) seedlings-
dc.typeArticle-
dc.citation.titleJournal of Plant Physiology-
dc.citation.number1-
dc.citation.endPage139-
dc.citation.startPage136-
dc.citation.volume157-
dc.contributor.affiliatedAuthorSuk Yoon Kwon-
dc.contributor.affiliatedAuthorHeang Soon Lee-
dc.contributor.affiliatedAuthorJang Ryol Liu-
dc.contributor.affiliatedAuthorSang Soo Kwak-
dc.contributor.alternativeName김재훈-
dc.contributor.alternativeName한수경-
dc.contributor.alternativeName권석윤-
dc.contributor.alternativeName이행순-
dc.contributor.alternativeName임용표-
dc.contributor.alternativeName유장렬-
dc.contributor.alternativeName곽상수-
dc.identifier.bibliographicCitationJournal of Plant Physiology, vol. 157, no. 1, pp. 136-139-
dc.identifier.doi10.1016/S0176-1617(00)80147-9-
dc.subject.keywordcucumis sativus-
dc.subject.keywordhypocotyl segment-
dc.subject.keywordplant regeneration-
dc.subject.keywordshoot induction-
dc.subject.localCucumis sativus-
dc.subject.localCucumis sativus L.-
dc.subject.localcucumis sativus-
dc.subject.localcucumis sativus L.-
dc.subject.localhypocotyl segment-
dc.subject.localPlant regeneration-
dc.subject.localplant regeneration-
dc.subject.localshoot induction-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.