DC Field | Value | Language |
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dc.contributor.author | Jae Whune Kim | - |
dc.contributor.author | Su Kyung Han | - |
dc.contributor.author | Suk Yoon Kwon | - |
dc.contributor.author | Heang Soon Lee | - |
dc.contributor.author | Yong Pyo Lim | - |
dc.contributor.author | Jang Ryol Liu | - |
dc.contributor.author | Sang Soo Kwak | - |
dc.date.accessioned | 2017-04-19T08:56:52Z | - |
dc.date.available | 2017-04-19T08:56:52Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 0176-1617 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/5009 | - |
dc.description.abstract | A method of high frequency shoot induction was established using excised hypocotyl segments bearing cotyledons (referred to as cotyledonary hypocotyl explants) of cucumber (Cucumis sativus L.) seedlings. Adventitious shoots were obtained from the upper excised hypocotyls of cucumber seedlings incubated on Murashige and Skoog (MS) medium containing 2.0 mg/L zeatin. The effects of cotyledon size and hypocotyl length of seedlings on shoot organogenesis were also examined. A higher frequency (80-90 %) of adventitious shoots was obtained from explants of 3- and 5-day-old seedlings with either 1 cotyledon or 2 half-cotyledons, and a short hypocotyl length (2 mm). However, explants with long hypocotyls and 2 cotyledons produced fewer shoots. Adventitious shoots from cotyledonary hypocotyl explants were removed and rooted in MS medium containing 1.0 mg/L IAA. The rooted plantlets developed into normal plants after acclimatization to ambient humidity levels. | - |
dc.publisher | Elsevier | - |
dc.title | High frequency shoot induction and plant regeneration from cotyledonary hypocotyl explants of Cucumber(Cucumis sativus L.) seedlings | - |
dc.title.alternative | High frequency shoot induction and plant regeneration from cotyledonary hypocotyl explants of Cucumber(Cucumis sativus L.) seedlings | - |
dc.type | Article | - |
dc.citation.title | Journal of Plant Physiology | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 139 | - |
dc.citation.startPage | 136 | - |
dc.citation.volume | 157 | - |
dc.contributor.affiliatedAuthor | Suk Yoon Kwon | - |
dc.contributor.affiliatedAuthor | Heang Soon Lee | - |
dc.contributor.affiliatedAuthor | Jang Ryol Liu | - |
dc.contributor.affiliatedAuthor | Sang Soo Kwak | - |
dc.contributor.alternativeName | 김재훈 | - |
dc.contributor.alternativeName | 한수경 | - |
dc.contributor.alternativeName | 권석윤 | - |
dc.contributor.alternativeName | 이행순 | - |
dc.contributor.alternativeName | 임용표 | - |
dc.contributor.alternativeName | 유장렬 | - |
dc.contributor.alternativeName | 곽상수 | - |
dc.identifier.bibliographicCitation | Journal of Plant Physiology, vol. 157, no. 1, pp. 136-139 | - |
dc.identifier.doi | 10.1016/S0176-1617(00)80147-9 | - |
dc.subject.keyword | cucumis sativus | - |
dc.subject.keyword | hypocotyl segment | - |
dc.subject.keyword | plant regeneration | - |
dc.subject.keyword | shoot induction | - |
dc.subject.local | Cucumis sativus | - |
dc.subject.local | Cucumis sativus L. | - |
dc.subject.local | cucumis sativus | - |
dc.subject.local | cucumis sativus L. | - |
dc.subject.local | hypocotyl segment | - |
dc.subject.local | Plant regeneration | - |
dc.subject.local | plant regeneration | - |
dc.subject.local | shoot induction | - |
dc.description.journalClass | Y | - |
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