DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jin Sung Lee | - |
dc.contributor.author | Jae Sam Hwang | - |
dc.contributor.author | Sang Mong Lee | - |
dc.contributor.author | Seung Hyun Sung | - |
dc.contributor.author | Yong Sung Kim | - |
dc.contributor.author | Dong Sang Suh | - |
dc.date.accessioned | 2017-04-19T08:56:52Z | - |
dc.date.available | 2017-04-19T08:56:52Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 1976-9571 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/5011 | - |
dc.description.abstract | To construct RFLP linkage map of silkworm (Bombyx mori), as a preliminary step, we assessed the efficiency for single or low copy sequences from silkworm genomic DNA. A genomic DNA plasmid library was constructed using EcoRI-digested 0.3 to 5 kb DNA fragments of II-111 strain. As results of two step screening (reverse colony hybridization and reverse southern hybridization) using a total genomic DNA as a probe, about 6% (70/1,200) of clones examined was estimated single or low copy sequences. At least 80% of total clones contained middle or high repetitive sequences. These results indicate that B. mori genome has a lot of high repetitive sequences. However, analysis for its precise copy number in the genome remains to be elucidated. The obtained 70 single-sequences will be useful molecular markers to study silkworm genomic analysis in the near future, although it has low efficiency of single copy sequence for constructing linkage map. | - |
dc.publisher | Springer | - |
dc.title | Development of RFLP markers from silkworm(bombyx mori) = 누에의 RFLP marker 개발 | - |
dc.title.alternative | Development of RFLP markers from silkworm(bombyx mori) | - |
dc.type | Article | - |
dc.citation.title | Genes & Genomics | - |
dc.citation.number | 4 | - |
dc.citation.endPage | 327 | - |
dc.citation.startPage | 319 | - |
dc.citation.volume | 21 | - |
dc.contributor.affiliatedAuthor | Yong Sung Kim | - |
dc.contributor.alternativeName | 이진성 | - |
dc.contributor.alternativeName | 황재삼 | - |
dc.contributor.alternativeName | 이상몽 | - |
dc.contributor.alternativeName | 성승현 | - |
dc.contributor.alternativeName | 김용성 | - |
dc.contributor.alternativeName | 서동상 | - |
dc.identifier.bibliographicCitation | Genes & Genomics, vol. 21, no. 4, pp. 319-327 | - |
dc.subject.keyword | linkage map | - |
dc.subject.keyword | plasmid genomic library | - |
dc.subject.keyword | RFLP | - |
dc.subject.keyword | silkworm | - |
dc.subject.local | linkage map | - |
dc.subject.local | plasmid genomic library | - |
dc.subject.local | RFLP | - |
dc.subject.local | RFLPs | - |
dc.subject.local | silkworm | - |
dc.subject.local | Silkworm | - |
dc.description.journalClass | Y | - |
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