Development of RFLP markers from silkworm(bombyx mori) = 누에의 RFLP marker 개발

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dc.contributor.authorJin Sung Lee-
dc.contributor.authorJae Sam Hwang-
dc.contributor.authorSang Mong Lee-
dc.contributor.authorSeung Hyun Sung-
dc.contributor.authorYong Sung Kim-
dc.contributor.authorDong Sang Suh-
dc.date.accessioned2017-04-19T08:56:52Z-
dc.date.available2017-04-19T08:56:52Z-
dc.date.issued1999-
dc.identifier.issn19769571-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5011-
dc.description.abstractTo construct RFLP linkage map of silkworm (Bombyx mori), as a preliminary step, we assessed the efficiency for single or low copy sequences from silkworm genomic DNA. A genomic DNA plasmid library was constructed using EcoRI-digested 0.3 to 5 kb DNA fragments of II-111 strain. As results of two step screening (reverse colony hybridization and reverse southern hybridization) using a total genomic DNA as a probe, about 6% (70/1,200) of clones examined was estimated single or low copy sequences. At least 80% of total clones contained middle or high repetitive sequences. These results indicate that B. mori genome has a lot of high repetitive sequences. However, analysis for its precise copy number in the genome remains to be elucidated. The obtained 70 single-sequences will be useful molecular markers to study silkworm genomic analysis in the near future, although it has low efficiency of single copy sequence for constructing linkage map.-
dc.publisherSpringer-
dc.titleDevelopment of RFLP markers from silkworm(bombyx mori) = 누에의 RFLP marker 개발-
dc.title.alternativeDevelopment of RFLP markers from silkworm(bombyx mori)-
dc.typeArticle-
dc.citation.titleGenes & Genomics-
dc.citation.number4-
dc.citation.endPage327-
dc.citation.startPage319-
dc.citation.volume21-
dc.contributor.affiliatedAuthorYong Sung Kim-
dc.contributor.alternativeName이진성-
dc.contributor.alternativeName황재삼-
dc.contributor.alternativeName이상몽-
dc.contributor.alternativeName성승현-
dc.contributor.alternativeName김용성-
dc.contributor.alternativeName서동상-
dc.identifier.bibliographicCitationGenes & Genomics, vol. 21, no. 4, pp. 319-327-
dc.subject.keywordlinkage map-
dc.subject.keywordplasmid genomic library-
dc.subject.keywordRFLP-
dc.subject.keywordsilkworm-
dc.subject.locallinkage map-
dc.subject.localplasmid genomic library-
dc.subject.localRFLP-
dc.subject.localsilkworm-
dc.subject.localSilkworm-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Genome Editing Research Center > 1. Journal Articles
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