Efficient establishment of presumptive embryonic stem cells from bovine blastocysts by exposure to calcium ionophore = Calcium ionophore를 이용한 소 배반포로 부터의 배아주 유사세포의 효과적인 분리

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Title
Efficient establishment of presumptive embryonic stem cells from bovine blastocysts by exposure to calcium ionophore = Calcium ionophore를 이용한 소 배반포로 부터의 배아주 유사세포의 효과적인 분리
Author(s)
Sun-Uk Kim; Zae Yoong Ryoo; Chul Sang Lee; Yong Mahn Han; Jung Sun Park; Dae Yeul Yu; Kyung Kwang Lee
Bibliographic Citation
Development & Reproduction, vol. 3, no. 1, pp. 45-51
Publication Year
1999
Abstract
It is crucial to remove trophectoderm (TE) cells of blastocysts for an efficient isolation of pluripotent embryonic stem (ES)-like cells from bovine blastocysts. We evaluated the effectiveness of chemosurgery using calcium ionophore A23l87 (CIPA) by investigating the viability and pluripotency of ES-like cell lines isolated from in vitro-produced bovine blastocysts after CIPA treatment. The blastocysts treated with 50 M CIPA for 25 min colonized most efficiently (51% of blastocysts) and developed to ES-like cell lines through 10 passages (4.8% of blastocysts) among CIPA-treated groups with different concentration and duration. In comparison with CIPA-untreated blastocysts, the colonization rate and overall viability of the CIPA-treated blastocysts were five times higher, suggesting that CIPA treatment condition defined in this study was highly efficient for establishing ES-like cell lines without apparent toxicity of CIPA. We evaluated in vitro pluripotency of the established three ES-like cell lines by examining alkaline phosphatase (AP) activity, capability of embryoid body formation, and chromosomal euploidity of the cells. Our cells showed a heterogeneous AP activity similarly to other reports. The cells were able to form simple embryoid bodies during suspension culture and majority of them showed a normal chromosome number of 60, the euploid chromosomal complement of bovine Therefore, our data suggest that CIPA treatment can be safely used for an efficient isolation of ES-like cell lines from bovine blastocysts.
Keyword
bovine blastocystcalcium ionophore A23187ES-like cell lines
ISSN
I000-0126
Publisher
South Korea
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
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