Characterization of KI-24, a novel murine monoclonal antibody with specific reactivity for the human immunodeficiency virus-1 p24 protein

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Title
Characterization of KI-24, a novel murine monoclonal antibody with specific reactivity for the human immunodeficiency virus-1 p24 protein
Author(s)
Song Yub Shin; Jung Hyun Park; Myung Kyu Lee; So Youn Jang; Kyung Soo Hahm
Bibliographic Citation
BMB Reports, vol. 33, no. 1, pp. 92-95
Publication Year
2000
Abstract
The HIV-1 p24(202-221) sequence ETINNEEEWDRVHPV HAGP contains a B-cell epitope with the earliest immune response and the highest antibody titer against anti-mouse sera obtained by immunization with p24 antigens. A novel mouse monoclonal antibody (mAb) was generated against the immunodominant B-cell epitope of the HIV-1 p24 capsid protein, p24(202-221). BALB/c mice were immunized with the four branched multiple antigenic peptide (MAP) containing the HIV-1 p24(202-221) sequence, and antibody-secreting hybridoma were produced by fusion of mouse splenocytes with P3X63Ag8.653, mouse myeloma cells. One clone which produced the antigen-specific mAb named KI-24 (Isotype IgG1, light chain: κ) was identified. mAb KI-24 was highly specific for both the p24(202-221) and p24 proteins when analyzed by ELISA and Western blotting. Since p24(202-221) also contains a cytotoxic T-lymphocyte epitope, this specfic peptide epitope and the monoclonal antibody with specific reactivity against the p24 protein and p24(202-221) can be used in peptide vaccine development and p24 antigen detection from HIV patients.
Keyword
B-cell epitopeHIV-1 p24(202-221)HIV-1 p24 proteinmonoclonal antibody
ISSN
1225-8687
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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