Improvement of intact human lipocortin-I production in Saccharomyces cerevisiae by inhibiting proteolysis

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Title
Improvement of intact human lipocortin-I production in Saccharomyces cerevisiae by inhibiting proteolysis
Author(s)
Won A Choi; Gui Hwan Oh; Kwang Hyun Ah; Bong Hyun Chung
Bibliographic Citation
Journal of Bioscience and Bioengineering, vol. 89, no. 1, pp. 77-80
Publication Year
2000
Abstract
Human lipocortin-I (hLC1), when was expressed as a secretory product in Saccharomyces cerevisiae, was cleaved to a significant extent by endoproteolytic processing, resulting in the accumulation of des1-26-hLC1 in the culture supernatant. This proteolytic cleavage was inhibited significantly by the addition of high concentrations of L-arginine and L- lysine, with a resultant marked improvement in the yield of intact hLC1. When the hLC1 was expressed in S. cerevisiae mutants deficient in one or two of the following endoproteases, Kex2p, Mke7p and Yps1p (Yap3p), the mutants exhibited no reduction in the extent of hLC1 proteolysis, indicating that these endoproteases are not involved in the proteolytic cleavage of hLC1.
Keyword
human lipocortin-IproteolysisL-lysineL- arginineendoproteasesSaccharomyces cerevisiae
ISSN
1389-1723
Publisher
Soc Bioscience Bioengineering Japan
DOI
http://dx.doi.org/10.1016/S1389-1723(00)88054-8
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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