Process development for production of recombinant human insulin-like growth factor-I in Escherichia coli

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dc.contributor.authorBong Hyun Chung-
dc.contributor.authorY J Choi-
dc.contributor.authorSung Ho Yoon-
dc.contributor.authorS Y Lee-
dc.contributor.authorYoung Ik Lee-
dc.description.abstractFed-batch cultures were carried out to overproduce human insulin-like growth factor I (IGF-I) in Escherichia coli. The effects of carbon sources (glucose or glycerol) and induction time on cell growth and IGF-I production were investigated in more detail. Glycerol was a better carbon source than glucose for IGF-I production in fed-batch culture. Induction at the mid- exponential phase with glycerol as a carbon source in the pH-stat fed-batch culture was optimal for IGF-I production. Under this condition, 2.8 g L-1 of fusion IGF-I was produced as inclusion bodies. We have also developed downstream processing for preparative scale purification of IGF-I from the fusion protein produced by the fed-batch culture using glycerol as a carbon source. After the fusion protein expressed was solubilized in 8 M urea and cleaved with hydroxylamine, the released IGF-I was purified by cation exchange chromatography, refolding and preparative scale reverse phase HPLC (rp-HPLC) to give recombinant IGF-I of >98% purity. The biological activities of the purified IGF-I were measured and found to be identical to those of commercial IGF-I.-
dc.titleProcess development for production of recombinant human insulin-like growth factor-I in Escherichia coli-
dc.title.alternativeProcess development for production of recombinant human insulin-like growth factor-I in Escherichia coli-
dc.citation.titleJournal of Industrial Microbiology & Biotechnology-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.affiliatedAuthorSung Ho Yoon-
dc.contributor.affiliatedAuthorYoung Ik Lee-
dc.identifier.bibliographicCitationJournal of Industrial Microbiology & Biotechnology, vol. 24, pp. 94-99-
dc.subject.keywordinsulin-like growth factor I (IGF-I)-
dc.subject.keywordfed-batch culture-
dc.subject.keyworddownstream processing-
dc.subject.keywordEscherichia coli-
dc.subject.localInsulin-like growth factor-I-
dc.subject.localinsulin-like growth factor I-
dc.subject.localinsulin-like growth factor type 1-
dc.subject.localInsulin-like growth factor-Ⅰ(IGF-Ⅰ)-
dc.subject.localinsulin-like growth factor I (IGF-I)-
dc.subject.localinsulin-like growth factor-Ⅰ(IGF-Ⅰ)-
dc.subject.localfed-batch culture-
dc.subject.localFed-batch culture-
dc.subject.localdownstream processing-
dc.subject.localEscherichia coli.-
dc.subject.localescherichia coli-
dc.subject.localEscherichia Coli-
dc.subject.localEscherichia coli-
dc.subject.localescherichia coil-
dc.subject.localE. coli-
dc.subject.localE. Coli-
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