DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ik Jae Moon | - |
dc.contributor.author | Young Ik Lee | - |
dc.contributor.author | Chun Sik Kwak | - |
dc.contributor.author | Je Ho Lee | - |
dc.contributor.author | Kyu Sam Choi | - |
dc.contributor.author | Alan D Schreiber | - |
dc.contributor.author | Jong Gu Park | - |
dc.date.accessioned | 2017-04-19T08:56:57Z | - |
dc.date.available | 2017-04-19T08:56:57Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/5048 | - |
dc.description.abstract | Systematic secondary structure simulation of a target mRNA sequence is shown to be effective for locating a good anti-sense target site. Multiple selected anti-sense sequences were placed in a single molecule. The anti-sense oligonucleotide (oligo) was covalently closed to avoid exonuclease activities and was designated CMAS (covalently closed multiple anti-sense)-oligo. CMAS-oligo was found to be stable, largely preserving its structural integrity after 24 h of incubation in the presence of either exonuclease III or serum. When human c-myb mRNA was targeted by the c-myb CMAS-oligo, expression of the gene was completely abolished. Further, tumour cell growth was inhibited by 82 ± 3% as determined by an MTT [3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] assay and by 90 ± 1% by [3H]thymidine incorporation. When a leukaemic cell line K562 was treated with CMAS-oligo, colony formation on soft agarose was also decreased by 93%. In contrast, treatment with a scrambled control oligo did not significantly inhibit leukaemic cell growth. These results suggest that a rational target site search is possible for an anti-sense oligo and that CMAS-oligo can be employed as an effective anti-sense agent with enhanced stability. | - |
dc.publisher | Portland Press Ltd | - |
dc.title | Target site search and effective inhibition of leukaemic cell growth by a covalently closed multiple anti-sense oligonucleotide to c-myb | - |
dc.title.alternative | Target site search and effective inhibition of leukaemic cell growth by a covalently closed multiple anti-sense oligonucleotide to c-myb | - |
dc.type | Article | - |
dc.citation.title | Biochemical Journal | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 303 | - |
dc.citation.startPage | 295 | - |
dc.citation.volume | 346 | - |
dc.contributor.affiliatedAuthor | Young Ik Lee | - |
dc.contributor.alternativeName | 문익재 | - |
dc.contributor.alternativeName | 이영익 | - |
dc.contributor.alternativeName | 곽춘식 | - |
dc.contributor.alternativeName | 이재호 | - |
dc.contributor.alternativeName | 최규삼 | - |
dc.contributor.alternativeName | Schreiber | - |
dc.contributor.alternativeName | 박종구 | - |
dc.identifier.bibliographicCitation | Biochemical Journal, vol. 346, no. 2, pp. 295-303 | - |
dc.identifier.doi | 10.1042/0264-6021:3460295 | - |
dc.subject.keyword | c-myb | - |
dc.subject.keyword | growth inhibition | - |
dc.subject.keyword | oligonucleotide stability | - |
dc.subject.local | c-myb | - |
dc.subject.local | Growth Inhibition | - |
dc.subject.local | growth inhibition | - |
dc.subject.local | Growth inhibition | - |
dc.subject.local | oligonucleotide stability | - |
dc.description.journalClass | Y | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.