Formation of α-D-glucose-1-phosphate by thermophilic α-1,4-D-glucan phosphorylase

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Title
Formation of α-D-glucose-1-phosphate by thermophilic α-1,4-D-glucan phosphorylase
Author(s)
Hyun Jae Shin; Y S Shin; Dae Sil Lee
Bibliographic Citation
Journal of Industrial Microbiology & Biotechnology, vol. 24, pp. 89-93
Publication Year
2000
Abstract
For the production of α-D-glucose-1-phosphate (G-1-P), α-1,4-D-glucan phosphorylase from Thermus caldophilus GK24 was partially purified to a specific activity of 13 U mg-1 and an enzyme recovery of 15%. The amount of G-1-P reached maximum (18%) when soluble starch was used as substrate, and the smallest substrate for G-1-P formation was maltotriose. The structure of purified G-1-P was confirmed by comparison to 13C-NMR data for an authentic sample. In addition to G-1-P, glucose-6-phosphate (12%) was simultaneously produced when 10 mM maltoheptaose was used as substrate.
Keyword
α-D-glucose-1-phosphateglucose-6-phosphateα-1,4-D-glucan phosphorylasephosphoglucomutasemaltooligosaccharidesThermus caldophilus GK24
ISSN
0169-4146
Publisher
Springer
DOI
http://dx.doi.org/10.1038/sj.jim.2900757
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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