Molecular cloning and characterization of the mouse Peroxiredoxin V gene

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Title
Molecular cloning and characterization of the mouse Peroxiredoxin V gene
Author(s)
Tae Hoon Lee; Sun Jung Kim; Sang Won Kang; Kyung Kwang Lee; Sue Goo Rhee; Dae Yeul Yu
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 270, no. 2, pp. 356-362
Publication Year
2000
Abstract
We have cloned two cDNA isoforms as well as genomic sequences of the mouse Prx V gene and characterized their molecular genetic features. Two isoforms of the mouse Prx V cDNA were identified from liver and testis. The testis-originated long transcripts had extra 1164-bp 5'-UTR sequences compared to the liver-originated short transcripts. Primer extension and sequence analyses revealed that the two isoforms were presumably transcribed at the same gene locus. The gene was composed of six exons spanning 3.2 kb. The short transcript was abundantly expressed in the kidney, liver, and heart of the adult mouse tissues and in the extra-membrane of the 10.5 dpc embryos. The long transcript of 1985 bp was abundantly detected in testis with trace amounts in other tissues. Interestingly, in testis and fetus, only mRNA expression of the long form was identified. However, the protein expression was not found in testis, implying that the long form could not properly direct the protein expression. The long Prx V cDNA has eight uORFs in the extra 5'-UTR, which proceed the major ORF. The inability of protein expression for the long-form cDNA in testis suggests that the uORFs might inhibit translation of the major ORF and thereby confer the tissue-specific regulation of the mouse Prx V gene.
Keyword
peroxiredoxin Vantioxidant enzymebacterial artificial chromosomeupstream open reading frame
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1006/bbrc.2000.2430
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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