Purification and characterization of extracellular medium-chain-length polyhydroxyalkanoate depolymerase from Pseudomomas sp. RY-1

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dc.contributor.authorHyun Mean Kim-
dc.contributor.authorKang Eun Ryu-
dc.contributor.authorKyung Sook Bae-
dc.contributor.authorYoung Ha Rhee-
dc.date.accessioned2017-04-19T08:56:58Z-
dc.date.available2017-04-19T08:56:58Z-
dc.date.issued2000-
dc.identifier.issn1389-1723-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5055-
dc.description.abstractA novel bacterial strain capable of growing in a medium containing a medium-chain-length polyhydroxyalkanoate (MCL-PHA) as the sole carbon source was isolated from a soil sample. The isolate, which was identified as Pseudomonas sp. RY-1, secreted MCL-PHA depolymerase into the culture fluid only when it was cultivated in a medium containing a MCL-PHA, such as polyhydroxyoctanoate (PHO) or polyhydroxynonanoate (PHN). The extracellular MCL-PHA depolymerase of this organism was purified to electrophoretic homogeneity. The enzyme was a tetramer with identical subunits and a total molecular mass of 115 kDa. The isoelectric point of this enzyme was estimated to be 5.9 by isoelectric focusing. The maximal activity was observed at pH 8.5 and 35°C. The enzyme was insensitive to phenylmethylsulfonyl fluoride and dithiothreitol, unlike other short-chain-length (SCL) PHA depolymerases. The K(m) values for PHO and PHN were 0.86 and 1.47 mg/ml, respectively. The enzyme could not hydrolyze SCL-PHAs and p-nitrophenyl esters of fatty acids.-
dc.publisherSoc Bioscience Bioengineering Japan-
dc.titlePurification and characterization of extracellular medium-chain-length polyhydroxyalkanoate depolymerase from Pseudomomas sp. RY-1-
dc.title.alternativePurification and characterization of extracellular medium-chain-length polyhydroxyalkanoate depolymerase from Pseudomomas sp. RY-1-
dc.typeArticle-
dc.citation.titleJournal of Bioscience and Bioengineering-
dc.citation.number2-
dc.citation.endPage198-
dc.citation.startPage196-
dc.citation.volume89-
dc.contributor.affiliatedAuthorKyung Sook Bae-
dc.contributor.alternativeName김현민-
dc.contributor.alternativeName류강은-
dc.contributor.alternativeName배경숙-
dc.contributor.alternativeName이영하-
dc.identifier.bibliographicCitationJournal of Bioscience and Bioengineering, vol. 89, no. 2, pp. 196-198-
dc.identifier.doi10.1016/S1389-1723(00)88737-X-
dc.subject.keywordMCL-PHA depolymerase-
dc.subject.keywordpolyhydroxyalkanoate-
dc.subject.keywordPseudomonas sp. RY-I-
dc.subject.localMCL-PHA depolymerase-
dc.subject.localPolyhydroxyalkanoate-
dc.subject.localPolyhydroxyalkanoates (PHAs)-
dc.subject.localPolyhydroxyalkanoates-
dc.subject.localPolyhydroxyalkonate-
dc.subject.localpolyhydroxyalkanoate-
dc.subject.localPseudomonas sp. RY-I-
dc.description.journalClassY-
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