A simplified procedure for the purification of human N-acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues
Cited 6 time in 
Metadata Downloads
Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Eun Young Song | - |
| dc.contributor.author | Kyoung A Kim | - |
| dc.contributor.author | Yung Dai Kim | - |
| dc.contributor.author | Dur Han Kwon | - |
| dc.contributor.author | Hong Soo Lee | - |
| dc.contributor.author | Cheorl Ho Kim | - |
| dc.contributor.author | Tae Wha Chung | - |
| dc.contributor.author | Si Myung Byun | - |
| dc.date.accessioned | 2017-04-19T08:56:58Z | - |
| dc.date.available | 2017-04-19T08:56:58Z | - |
| dc.date.issued | 2000 | - |
| dc.identifier.issn | 0141-5492 | - |
| dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/5057 | - |
| dc.description.abstract | Human N-acetylglucosaminyltransferase-III (GnT-III) has not been hitherto purified from either tissue or cell line. Although rat GnT-III has been purified from rat kidney tissue, the procedure involves laborious and complex steps. We have developed a simplified procedure based on a QAE (diethyl[2-hydroxypropyl]aminoethyl)-Sepharose and an immunoaffinity chromatography technique. The immunoaffinity chromatography utilized a monoclonal antibody to human aglycosyl recombinant N- acetylglucosaminyltransferase-III. With the new procedure, human N- acetylglucosaminyltransferase-III was purified from hepatocellular carcinoma tissues with an increase in the specific enzyme activity of 378-fold. | - |
| dc.publisher | Springer | - |
| dc.title | A simplified procedure for the purification of human N-acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues | - |
| dc.title.alternative | A simplified procedure for the purification of human N-acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues | - |
| dc.type | Article | - |
| dc.citation.title | Biotechnology Letters | - |
| dc.citation.number | 3 | - |
| dc.citation.endPage | 204 | - |
| dc.citation.startPage | 201 | - |
| dc.citation.volume | 22 | - |
| dc.contributor.affiliatedAuthor | Eun Young Song | - |
| dc.contributor.affiliatedAuthor | Yung Dai Kim | - |
| dc.contributor.affiliatedAuthor | Dur Han Kwon | - |
| dc.contributor.affiliatedAuthor | Hong Soo Lee | - |
| dc.contributor.alternativeName | 송은영 | - |
| dc.contributor.alternativeName | 김경아 | - |
| dc.contributor.alternativeName | 김영대 | - |
| dc.contributor.alternativeName | 권두한 | - |
| dc.contributor.alternativeName | 이홍수 | - |
| dc.contributor.alternativeName | 김철호 | - |
| dc.contributor.alternativeName | 정태화 | - |
| dc.contributor.alternativeName | 변시명 | - |
| dc.identifier.bibliographicCitation | Biotechnology Letters, vol. 22, no. 3, pp. 201-204 | - |
| dc.identifier.doi | 10.1023/A:1005641115696 | - |
| dc.subject.keyword | hepatocellular carcinoma | - |
| dc.subject.keyword | immunoaffinity column | - |
| dc.subject.keyword | monoclonal antibody | - |
| dc.subject.keyword | N-acetylglucosaminyltransferase | - |
| dc.subject.local | Hepatocellular carcinoma | - |
| dc.subject.local | Hepatocellular carcinoma (HCC) | - |
| dc.subject.local | Hepatocellular carcinomas | - |
| dc.subject.local | hepatocellular carcinoma | - |
| dc.subject.local | hepatocellular carcinoma (HCC) | - |
| dc.subject.local | immunoaffinity column | - |
| dc.subject.local | monoclonal antibodies | - |
| dc.subject.local | monoclonal antibody | - |
| dc.subject.local | Monoclonal Antibodies | - |
| dc.subject.local | Monoclonal Antibody | - |
| dc.subject.local | Monoclonal antibodies | - |
| dc.subject.local | Monoclonal antibody | - |
| dc.subject.local | Monoclonal antibody (mAb) | - |
| dc.subject.local | N-acetylglucosaminyltransferase | - |
| dc.description.journalClass | Y | - |
- Appears in Collections:
- 1. Journal Articles > Journal Articles
- Files in This Item:
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.