Germ-line transmission of a targeted duplication made in a renin gene by gap-repair in embryonic stem cells = 배아간세포에서 간극수복(gap-repair)에 의해 중복된 레닌유전자의 배선세포로의 전달

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Title
Germ-line transmission of a targeted duplication made in a renin gene by gap-repair in embryonic stem cells = 배아간세포에서 간극수복(gap-repair)에 의해 중복된 레닌유전자의 배선세포로의 전달
Author(s)
Byung Jae Lee; Goo Taeg Oh
Bibliographic Citation
Korean Journal of Laboratory Animal Science, vol. 16, no. 2, pp. 149-157
Publication Year
2000
Abstract
Embryonic stem cells (derived from 129/Ola mice) containing a mutant hypoxanthine phosphoribosyltransferase gene that had been corrected in vitro in a planned manner by homologous recombination were injected into blastocysts obtained from C57BL/6J mice. The injected blastocysts were introduced into pseudopregnant female mice to complete their development. Eleven surviving pups were obtained. Nine were chimeras: six males and three females. Two of the males transmitted the embryonic stem cell genome containing the alteration in the hypoxanthine phosphoribosyltransferase gene to their offspring at high frequencies. These experiments demonstrate that a preplanned alteration in a chosen gene can be made in the germ line of an experimental animal by homologous recombination in an embryonic stem cell.
Keyword
renin genegene duplicationgap-repairembryonic stem cell
ISSN
1225-813X
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.1073/pnas.86.22.8927
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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