Involvement of meiotic maturation of Oocytes in promoting granulosa cell proliferation

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Title
Involvement of meiotic maturation of Oocytes in promoting granulosa cell proliferation
Author(s)
Kyu Tae Chang; Dong Un Han; Tae Yoon Kim; Dong Soo Son; Mun Il Kang; Byung Hwa Hyun; Byoung Gil Mheen
Bibliographic Citation
Journal of Reproduction and Development, vol. 46, no. 3, pp. 139-145
Publication Year
2000
Abstract
The effect of porcine oocytes on the proliferation of rat granulosa cells was studied in a heterologous culture system. Morphologically high quality oocytes showed a more potent stimulatory effect on DNA synthesis in granulosa cells than low quality oocytes. This stimulatory effect of porcine oocytes was enhanced by FSH and cAMP, but was unaffected by both transforming growth factor-β and tumor necrosis factor-oc. Maturation of porcine oocytes in vitro attenuated the stimulatory effect of FSH and cAMP on granulosa cell DNA synthesis. In addition, rat oocytes also exhibited a similar facilitatory effect on DNA synthesis in rat granulosa cells. These results suggest that a soluble factor(s) secreted from oocytes, the secretion of which is dependent on their meiotic progression, can stimulate proliferation of rat granulosa cells in a heterologous culture system. The molecular nature of the factor(s) remains to be elucidated.
Keyword
oocytegranulosa cellFSHTGF-βTNF-α
ISSN
I000-0068
Publisher
Society Reproduction & Development-Srd
DOI
http://dx.doi.org/10.1262/jrd.46.139
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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