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- Title
- Antiphotooxidative activity of protoberberines derived from coptis japonica makino in the chlorophyll-sensitized photooxidation of oil
- Author(s)
- Jin Pyo Kim; Mun Yhung Jung; Jong Pyung Kim; Sung Yeol Kim
- Bibliographic Citation
- Journal of Agricultural and Food Chemistry, vol. 48, no. 4, pp. 1058-1063
- Publication Year
- 2000
- Abstract
- Antiphotooxidative components were isolated from the methanolic extract of Coptis japonica Makino by liquid-liquid partitioning fractionation, subsequent column chromatography on Sephadex LH20 and silica gel, and preparative silica gel TLC. The isolated compounds were identified as coptisine, jatrorrizhine, berberine, and magnoflorine by a combination of spectroscopic studies using UV-visible, IR, mass-spectrometry, and NMR. Coptisine, jatrorrizhine, and berberine isolated from Coptis japonica Makino showed strong antiphotooxidative activity in the chlorophyll-sensitized photooxidation of linoleic acid. However, these compounds did not show either inhibitory activity against lipid peroxidation in rat liver microsomes nor DPPH radical scavenging activity, indicating that their antiphotooxidative activity was not due to the radical chain reaction breaking ability but due to singlet oxygen quenching activity. Commercially available authentic protoberberines (berberine chloride and palmatine chloride) also showed strong antioxidative activity in the chlorophyllsensitized photooxidation of linoleic acid. The antiphotooxidative activities of the berberine chloride and palmatine chloride were significantly higher than that of ascorbyl palmitate in the chlorophyllsensitized photooxidation of linoleic acid. These results clearly showed for the first time the antiphotooxidative properties of protoberberines in chlorophyll-sensitized photooxidation of oil.
- Keyword
- Coptis japonica Makinophotooxidationberberinecoptisinejatrorrizhinemagnoflorinechlorophyllssinglet oxygen
- ISSN
- 0021-8561
- Publisher
- Amer Chem Soc
- DOI
- http://dx.doi.org/10.1021/jf9909297
- Type
- Article
- Appears in Collections:
- Ochang Branch Institute > Natural Product Research Center > 1. Journal Articles
- Files in This Item:
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